1) modified CTAB-LiCl
改良CTAB-LiCl法
1.
The results showed that the modified CTAB-LiCl was capable of efficiently removing polysaccharides,and that of the RNAs extracted by the modified CTAB-LiCl,the lightness of 28 s rRNA was two times as much as that of 18 S Rrna, the value of A260/A280,A260/A230 was1.
结果表明,改良CTAB-LiCl法能有效地去除多糖,提取到的RNA 28 SrRNA亮度约为18SrRNA的二倍,A260/A280比值为1。
2) modified CTAB method
改良CTAB法
1.
Methods: Two different methods,conventional CTAB method and modified CTAB method,were applied to isolate genomic DNA from dry leaves of Lonicera Japonica Thunb.
方法:分别采用常规CTAB法与改良CTAB法提取干叶的DNA,通过琼脂糖凝胶电泳、紫外分光光度法、酶切检测其质量,并与鲜叶的提取效果比较。
2.
To satisfy their increasing researches in molecular Biology, modified CTAB and different concentration gradient of EDTA with 2-mercaptoethanol in modified CTAB method are described in the present paper.
采用改良CTAB法以及不同 2 -巯基乙醇梯度和不同EDTA梯度分离基因组DNA的方法对多糖含量高、极易褐化的美味猕猴桃嫩叶基因组DNA提取条件进行了优化。
3.
Method:Two different methods,conventional CTAB method and modified CTAB method,were applied to extract genomic DNA from leaves of Lonicera japonica Thunb.
方法:采用改良CTAB法从忍冬叶片中提取DNA,通过琼脂糖凝胶电泳、紫外分光光度法、酶切检测其质量,并与常规CTAB法比较。
3) Improved CTAB Method
改良CTAB法
1.
The result showed that DNAs extracted by the improved CTAB method are much purer than those of SDS method.
采用改良CTAB法和SDS法提取椰子基因组DNA,并通过琼脂糖凝胶电泳、分光光度计进行紫外检测。
2.
Method: The improved CTAB method and improved SDS method were used to extract DNA.
方法:采用改良CTAB法和改良SDS法提取澳洲坚果样品的总DNA,并对产物进行紫外、电泳及PCR扩增检测。
3.
By studying the appearance,concentration,A260/A280,A260/A230 and electrophoresis,and comparing the total DNA extracted from ISSR analysis,the result showed that the improved CTAB method was suitable for ISSR analysis,and it was a better DNA extraction method of Casta nopsis fissa.
对大叶栎的总DNA两种提取方法进行对比试验,从外观、浓度、A260/A280、A260/A230、电泳检查和ISSR扩增对所提取到的DNA进行对比,结果显示用改良CTAB法提取的DNA在各方面都达到ISSR扩增的要求,是大叶栎总DNA提取的较好方法。
4) CTAB-LiCl method
CTAB-LiCl法
1.
The results show the higher yield and good quality of RNA are extracted using an improved CTAB-LiCl method which can be used directly in cDNA synthesis, cDNA-AFLP analysis and other molecular biological experiment.
试验以茶树种胚为研究对象,通过对几种提取总RNA方法的比较与分析,认为用改进的CTAB-LiCl法提取茶树种胚总RNA的产率较高,质量好,可直接用于cDNA的合成、cDNA-AFLP分析等分子生物学实验操作。
2.
Total RNA extracted by CTAB-LiCl method had high quality, which is the best method of total RNA isolation from Dioscorea opposita.
采用异硫氰酸胍-巯基乙醇联合变性法、Trizol法和CTAB-LiCl法等3种方法,分别对山药叶片和块茎进行总RNA提取效果进行了比较。
5) improved CTAB method
改良的CTAB法
1.
To find a normal method for extracting genomic DNA from planaria, the improved CTAB method, SDS method, SDS-Proteinase K method were used to extract genomic DNA from Dugesia japonica.
为了找到一种适用于提取涡虫基因组DNA的常规方法,我们以东亚三角头涡虫为材料,分别用改良的CTAB法、SDS法、SDS-蛋白酶K法对涡虫的基因组DNA进行了制备,并对3种方法制备的涡虫基因组DNA进行了检测与比较。
2.
The experiment results showed that the improved CTAB method was suitable for all kinds of molecular analysis due to its simplicity and high efficiency.
采用改良的CTAB法,植物基因组DNA小量制备试剂盒,SDS法和高盐低pH值法4种方法提取安徽市场上8种大豆种子的基因组DNA,首次用降落PCR(TD-PCR)检测转基因成分,并对阳性产物进行酶切验证。
6) CTAB-LiCl-based extraction method
CTAB-LiCl提取法
1.
A novel and simple CTAB-LiCl-based extraction method for high-quality and total RNA of rice embryo samples is developed.
介绍了一种全新、简单的提取水稻种胚RNA的CTAB-LiCl提取法,可在不用液氮的室温下有效地排除剥取的种胚中大量带有的多糖和本身脂质的干扰,所得RNA样品经紫外分光光度计和琼脂糖凝胶电泳分析证明具有较高的纯度和完整性,并可进一步满足RT-PCR和Northern印迹的实验需要,适用于富含多糖和脂质的植物组织RNA的提取。
补充资料：改良
①去掉事物的个别缺点，使更适合要求：～土壤 ㄧ～品种。②改善。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条