1) SDS-LiCl improved method
SDS-LiCl改进法
1.
japonicus leaves as material, the effect of total RNA extraction using different methods of guanidine thiocyanate method,SDS-LiCl method and SDS-LiCl improved method was compared and the influence of the active carbon and ascorbic acid on the quality of RNA extraction was discussed.
[方法]以百脉根叶片为材料,比较异硫氰酸胍法、SDS-LiCl法及SDS-LiCl改进法提取总RNA的效果并探讨活性炭、抗坏血酸对RNA提取质量的影响。
2) SDS-LiCl method
SDS-LiCl法
1.
Method: Several commercialized or commonly used methods,such as RNA Plant Reagent(Tiangen),Plant RNA Purification Reagent(Invitrogen),UNIQ column total RNA isolation kit(Sangon),SDS-LiCl method and TRIZOL Reagent(Invitrogen),etc.
方法:以灰绿藜叶片为材料,应用RNAPlant Reagent法(Tiangen)、Plant RNA purification Reagent法(Invitrogen)、UNIQ柱式总RNA试剂盒提取法(Sangon)、SDS-LiCl法、TRIZOL试剂法(Invitrogen)等进行总RNA的提取,并构建其全长cDNA文库和抑制消减文库。
2.
hispida in the four species,while the SDS-LiCl method failed to extract total RNA from F.
用Trizol法、SDS-LiCl法、改良CTAB法分别对对叶榕(Ficus hispida)、聚果榕(F。
3) modified CTAB-LiCl
改良CTAB-LiCl法
1.
The results showed that the modified CTAB-LiCl was capable of efficiently removing polysaccharides,and that of the RNAs extracted by the modified CTAB-LiCl,the lightness of 28 s rRNA was two times as much as that of 18 S Rrna, the value of A260/A280,A260/A230 was1.
结果表明,改良CTAB-LiCl法能有效地去除多糖,提取到的RNA 28 SrRNA亮度约为18SrRNA的二倍,A260/A280比值为1。
4) improved SDS method
改良SDS法
1.
Method: The improved CTAB method and improved SDS method were used to extract DNA.
方法:采用改良CTAB法和改良SDS法提取澳洲坚果样品的总DNA,并对产物进行紫外、电泳及PCR扩增检测。
2.
Traditional SDS method was simplified and improved,improved SDS method was found,DNA which was extracted from root tips of peanut by improved SDS method was high quality and was satisfied for molecular biology research.
对传统的SDS法进行简化和优化,建立了改良SDS法,利用改良SDS法提取花生根尖DNA,效果较好,能够满足分子生物学研究需要。
5) Modified SDS-CTAB method
改进的SDS-CTAB
6) LiCl method
LiCl法
1.
To look for rapid and efficient methods to isolate DNA,three algae(Porphyra yezoensis,Enteromorpha clathrata and Spirulina platensis)were as materials to extract DNA by three preparation methods,improved CTAB method,LiCl method and extraction kit.
选取三种不同海藻纲中的条斑紫菜、条浒苔、钝顶螺旋藻为材料,通过采用改良的CTAB法、LiCl法及试剂盒法抽提基因组DNA并进行比较,以找出适用于各种藻类DNA提取的快速有效方法。
补充资料：超改进控制加权形心单纯形法
分子式：
CAS号：
性质：结合了超改进单纯形法与控制形心单纯形法两者的优点而产生的一种单纯形优化方法。由除响应值最差的顶点w以外的其余各单纯形顶点的响应值计算加权形心点pw，引入控制参数γ调节加权形心点的位置，确定w的反射点pγ，以保证单纯形沿逼近梯度的方向推移。在确定的推移方向上，根据顶点w、加权形心点pw。和反射点pγ的响应值用二次曲线拟合求导，决定单纯形的新顶点，以保证新顶点在该推移方向上是最优的，从而加快了优化速度。
CAS号：
性质：结合了超改进单纯形法与控制形心单纯形法两者的优点而产生的一种单纯形优化方法。由除响应值最差的顶点w以外的其余各单纯形顶点的响应值计算加权形心点pw，引入控制参数γ调节加权形心点的位置，确定w的反射点pγ，以保证单纯形沿逼近梯度的方向推移。在确定的推移方向上，根据顶点w、加权形心点pw。和反射点pγ的响应值用二次曲线拟合求导，决定单纯形的新顶点，以保证新顶点在该推移方向上是最优的，从而加快了优化速度。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条