1) modified CTAB
改良CTAB
1.
This experiment made use of the modified CTAB,carried on Mararate isolation and purification of DNA from various corn samples.
利用改良CTAB法提取玉米产品的基因组DNA,应用PCR检测方法,并以玉米特异性内源基因IVR为内参,花椰菜花叶病毒35S启动子(CaMV35S启动子)、农杆菌胭脂碱合成酶终止子(NOS终止子)为靶基因检测玉米中是否存在转基因成分。
2.
Experiment was carried out on isolation of total RNA from flower-bud of Carya cathayensis by the methods of modified CTAB,guanidine isothiocyanate and modified hot phenol.
以山核桃的花芽为材料,利用改良CTAB法、异硫氰酸胍法和改良热酚法提取其总RNA,并对RNA产率、纯度、电泳图谱和RT-PCR进行分析,结果表明:改良CTAB法明显优于其它两种提取方法,用它提取的28S rRNA、18S rRNA条带清晰、稳定,无降解,OD260/OD280值保持在1。
2) modified CTAB method
改良CTAB法
1.
Methods: Two different methods,conventional CTAB method and modified CTAB method,were applied to isolate genomic DNA from dry leaves of Lonicera Japonica Thunb.
方法:分别采用常规CTAB法与改良CTAB法提取干叶的DNA,通过琼脂糖凝胶电泳、紫外分光光度法、酶切检测其质量,并与鲜叶的提取效果比较。
2.
To satisfy their increasing researches in molecular Biology, modified CTAB and different concentration gradient of EDTA with 2-mercaptoethanol in modified CTAB method are described in the present paper.
采用改良CTAB法以及不同 2 -巯基乙醇梯度和不同EDTA梯度分离基因组DNA的方法对多糖含量高、极易褐化的美味猕猴桃嫩叶基因组DNA提取条件进行了优化。
3.
Method:Two different methods,conventional CTAB method and modified CTAB method,were applied to extract genomic DNA from leaves of Lonicera japonica Thunb.
方法:采用改良CTAB法从忍冬叶片中提取DNA,通过琼脂糖凝胶电泳、紫外分光光度法、酶切检测其质量,并与常规CTAB法比较。
3) Improved CTAB Method
改良CTAB法
1.
The result showed that DNAs extracted by the improved CTAB method are much purer than those of SDS method.
采用改良CTAB法和SDS法提取椰子基因组DNA,并通过琼脂糖凝胶电泳、分光光度计进行紫外检测。
2.
Method: The improved CTAB method and improved SDS method were used to extract DNA.
方法:采用改良CTAB法和改良SDS法提取澳洲坚果样品的总DNA,并对产物进行紫外、电泳及PCR扩增检测。
3.
By studying the appearance,concentration,A260/A280,A260/A230 and electrophoresis,and comparing the total DNA extracted from ISSR analysis,the result showed that the improved CTAB method was suitable for ISSR analysis,and it was a better DNA extraction method of Casta nopsis fissa.
对大叶栎的总DNA两种提取方法进行对比试验,从外观、浓度、A260/A280、A260/A230、电泳检查和ISSR扩增对所提取到的DNA进行对比,结果显示用改良CTAB法提取的DNA在各方面都达到ISSR扩增的要求,是大叶栎总DNA提取的较好方法。
4) improved CTAB method
改良的CTAB法
1.
To find a normal method for extracting genomic DNA from planaria, the improved CTAB method, SDS method, SDS-Proteinase K method were used to extract genomic DNA from Dugesia japonica.
为了找到一种适用于提取涡虫基因组DNA的常规方法,我们以东亚三角头涡虫为材料,分别用改良的CTAB法、SDS法、SDS-蛋白酶K法对涡虫的基因组DNA进行了制备,并对3种方法制备的涡虫基因组DNA进行了检测与比较。
2.
The experiment results showed that the improved CTAB method was suitable for all kinds of molecular analysis due to its simplicity and high efficiency.
采用改良的CTAB法,植物基因组DNA小量制备试剂盒,SDS法和高盐低pH值法4种方法提取安徽市场上8种大豆种子的基因组DNA,首次用降落PCR(TD-PCR)检测转基因成分,并对阳性产物进行酶切验证。
5) modified CTAB-LiCl
改良CTAB-LiCl法
1.
The results showed that the modified CTAB-LiCl was capable of efficiently removing polysaccharides,and that of the RNAs extracted by the modified CTAB-LiCl,the lightness of 28 s rRNA was two times as much as that of 18 S Rrna, the value of A260/A280,A260/A230 was1.
结果表明,改良CTAB-LiCl法能有效地去除多糖,提取到的RNA 28 SrRNA亮度约为18SrRNA的二倍,A260/A280比值为1。
6) modified CTAB method
改进CTAB法
1.
Methods: Genomic DNA was extracted by classical CTAB method,modified CTAB method with the use of lysozyme and UniQ ion-exchange method,the quality and quantity of genomic DNA were compared.
方法:用经典CTAB提取法、改进CTAB法(溶菌酶处理结合CTAB提取法)、UniQ柱吸附提取法制备苛求芽孢杆菌基因组DNA,比较产物完整性和用于PCR扩增的有效性。
补充资料：acetoquat ctab
CAS：57-09-0
分子式：C19H42BrN
分子质量：364.45
熔点：250-237℃
中文名称：十六烷基三甲基溴化铵
N,N,N-三甲基-1-十六烷基溴化铵
鲸熔三甲基溴化铵
阳性皂
英文名称：Hexadecyltrimethyl ammonium bromide
Cetyltrimethylammonium bromide
hexadecyltrimethyl-ammoniu bromide
(1-hexadecyl)trimethylammonium bromide
n,n,n-trimethyl-1-hexadecanaminiu bromide
acetoquat ctab
性状描述：白色微晶形粉末。熔点>230℃（分解）。溶于水，微溶于醇，不溶于醚。水溶液呈碱性，产生大量气泡，具刺鼻臭味。
生产方法：由十六醇经溴素溴化后与三甲胺反应生成季铵盐。
用途：用于有机合成，也可作为阳离子表面活性剂，用作杀菌剂，配制含漱剂、消毒剂，肠内葡萄糖吸收抑制剂。该品也是常用的胶束增溶分光光度法试剂。
分子式：C19H42BrN
分子质量：364.45
熔点：250-237℃
中文名称：十六烷基三甲基溴化铵
N,N,N-三甲基-1-十六烷基溴化铵
鲸熔三甲基溴化铵
阳性皂
英文名称：Hexadecyltrimethyl ammonium bromide
Cetyltrimethylammonium bromide
hexadecyltrimethyl-ammoniu bromide
(1-hexadecyl)trimethylammonium bromide
n,n,n-trimethyl-1-hexadecanaminiu bromide
acetoquat ctab
性状描述：白色微晶形粉末。熔点>230℃（分解）。溶于水，微溶于醇，不溶于醚。水溶液呈碱性，产生大量气泡，具刺鼻臭味。
生产方法：由十六醇经溴素溴化后与三甲胺反应生成季铵盐。
用途：用于有机合成，也可作为阳离子表面活性剂，用作杀菌剂，配制含漱剂、消毒剂，肠内葡萄糖吸收抑制剂。该品也是常用的胶束增溶分光光度法试剂。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条