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1)  LiCl method
LiCl法
1.
To look for rapid and efficient methods to isolate DNA,three algae(Porphyra yezoensis,Enteromorpha clathrata and Spirulina platensis)were as materials to extract DNA by three preparation methods,improved CTAB method,LiCl method and extraction kit.
选取三种不同海藻纲中的条斑紫菜、条浒苔、钝顶螺旋藻为材料,通过采用改良的CTAB法、LiCl法及试剂盒法抽提基因组DNA并进行比较,以找出适用于各种藻类DNA提取的快速有效方法。
2)  LiCl precipitating method
LiCl沉淀法
1.
But capsules, especially capsule polysaccharides, will disturb DNA extracting largely, thus, LiCl precipitating method was introduced in this paper as a novel method and was examined by electrophoresis, spectrophotometer and amplification of 16S rRNA gene.
结合提取基因组DNA的CTAB法(hexadecyltrimethylammoniumbromide,十六烷基三甲基溴化铵)和用于提取海藻基因组DNA的LiCl法,设计了针对产生较多荚膜多糖细菌的DNA提取方法—LiCl沉淀法。
3)  Urea-LiCl method
尿素-LiCl法
4)  CTAB-LiCl method
CTAB-LiCl法
1.
The results show the higher yield and good quality of RNA are extracted using an improved CTAB-LiCl method which can be used directly in cDNA synthesis, cDNA-AFLP analysis and other molecular biological experiment.
试验以茶树种胚为研究对象,通过对几种提取总RNA方法的比较与分析,认为用改进的CTAB-LiCl法提取茶树种胚总RNA的产率较高,质量好,可直接用于cDNA的合成、cDNA-AFLP分析等分子生物学实验操作。
2.
Total RNA extracted by CTAB-LiCl method had high quality, which is the best method of total RNA isolation from Dioscorea opposita.
采用异硫氰酸胍-巯基乙醇联合变性法、Trizol法和CTAB-LiCl法等3种方法,分别对山药叶片和块茎进行总RNA提取效果进行了比较。
5)  SDS-LiCl method
SDS-LiCl法
1.
Method: Several commercialized or commonly used methods,such as RNA Plant Reagent(Tiangen),Plant RNA Purification Reagent(Invitrogen),UNIQ column total RNA isolation kit(Sangon),SDS-LiCl method and TRIZOL Reagent(Invitrogen),etc.
方法:以灰绿藜叶片为材料,应用RNAPlant Reagent法(Tiangen)、Plant RNA purification Reagent法(Invitrogen)、UNIQ柱式总RNA试剂盒提取法(Sangon)、SDS-LiCl法、TRIZOL试剂法(Invitrogen)等进行总RNA的提取,并构建其全长cDNA文库和抑制消减文库。
2.
hispida in the four species,while the SDS-LiCl method failed to extract total RNA from F.
用Trizol法、SDS-LiCl法、改良CTAB法分别对对叶榕(Ficus hispida)、聚果榕(F。
6)  SDS-LiCl improved method
SDS-LiCl改进法
1.
japonicus leaves as material, the effect of total RNA extraction using different methods of guanidine thiocyanate method,SDS-LiCl method and SDS-LiCl improved method was compared and the influence of the active carbon and ascorbic acid on the quality of RNA extraction was discussed.
[方法]以百脉根叶片为材料,比较异硫氰酸胍法、SDS-LiCl法及SDS-LiCl改进法提取总RNA的效果并探讨活性炭、抗坏血酸对RNA提取质量的影响。
补充资料:licl
CAS:7447-41-8
分子式:ClLi
分子质量:42.39
中文名称:氯化锂;氯化锂(无水);氯化锂(结晶);氯化锂,无水
英文名称:Lithium chloride;Lithium chloride, anhydrous;chlorku litu;chlorure de lithium;licl;lithium chloride (licl);Lithiumchlorid
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
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