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1)  modified CTAB method
改进CTAB法
1.
Methods: Genomic DNA was extracted by classical CTAB method,modified CTAB method with the use of lysozyme and UniQ ion-exchange method,the quality and quantity of genomic DNA were compared.
方法:用经典CTAB提取法、改进CTAB法(溶菌酶处理结合CTAB提取法)、UniQ柱吸附提取法制备苛求芽孢杆菌基因组DNA,比较产物完整性和用于PCR扩增的有效性。
2)  the improved CTAB method
CTAB改进法
3)  modified CTAB method
改进的CTAB法
1.
The results showed that the modified CTAB method was better than regular method in terms of the quality of total DNA extraction.
用改进的CTAB法提取猪毛菜、碱蓬、骆驼蓬、骆驼蹄瓣和柽柳5种盐生植物基因组DNA,通过与常规的CTAB法提取结果相比发现,改进的CTAB法从提取速度、得率、质量上都优于常规的CTAB法,并总结出一套适用于盐生植物的快速高质量基因组DNA的提取方法。
4)  modified CTAB method
改良CTAB法
1.
Methods: Two different methods,conventional CTAB method and modified CTAB method,were applied to isolate genomic DNA from dry leaves of Lonicera Japonica Thunb.
方法:分别采用常规CTAB法与改良CTAB法提取干叶的DNA,通过琼脂糖凝胶电泳、紫外分光光度法、酶切检测其质量,并与鲜叶的提取效果比较。
2.
To satisfy their increasing researches in molecular Biology, modified CTAB and different concentration gradient of EDTA with 2-mercaptoethanol in modified CTAB method are described in the present paper.
采用改良CTAB法以及不同 2 -巯基乙醇梯度和不同EDTA梯度分离基因组DNA的方法对多糖含量高、极易褐化的美味猕猴桃嫩叶基因组DNA提取条件进行了优化。
3.
Method:Two different methods,conventional CTAB method and modified CTAB method,were applied to extract genomic DNA from leaves of Lonicera japonica Thunb.
方法:采用改良CTAB法从忍冬叶片中提取DNA,通过琼脂糖凝胶电泳、紫外分光光度法、酶切检测其质量,并与常规CTAB法比较。
5)  Improved CTAB Method
改良CTAB法
1.
The result showed that DNAs extracted by the improved CTAB method are much purer than those of SDS method.
采用改良CTAB法和SDS法提取椰子基因组DNA,并通过琼脂糖凝胶电泳、分光光度计进行紫外检测。
2.
Method: The improved CTAB method and improved SDS method were used to extract DNA.
方法:采用改良CTAB法和改良SDS法提取澳洲坚果样品的总DNA,并对产物进行紫外、电泳及PCR扩增检测。
3.
By studying the appearance,concentration,A260/A280,A260/A230 and electrophoresis,and comparing the total DNA extracted from ISSR analysis,the result showed that the improved CTAB method was suitable for ISSR analysis,and it was a better DNA extraction method of Casta nopsis fissa.
对大叶栎的总DNA两种提取方法进行对比试验,从外观、浓度、A260/A280、A260/A230、电泳检查和ISSR扩增对所提取到的DNA进行对比,结果显示用改良CTAB法提取的DNA在各方面都达到ISSR扩增的要求,是大叶栎总DNA提取的较好方法。
6)  Modified SDS-CTAB method
改进的SDS-CTAB
补充资料:超改进控制加权形心单纯形法
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CAS号:

性质:结合了超改进单纯形法与控制形心单纯形法两者的优点而产生的一种单纯形优化方法。由除响应值最差的顶点w以外的其余各单纯形顶点的响应值计算加权形心点pw,引入控制参数γ调节加权形心点的位置,确定w的反射点pγ,以保证单纯形沿逼近梯度的方向推移。在确定的推移方向上,根据顶点w、加权形心点pw。和反射点pγ的响应值用二次曲线拟合求导,决定单纯形的新顶点,以保证新顶点在该推移方向上是最优的,从而加快了优化速度。

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