1) SDS-LiCl method
SDS-LiCl法
1.
Method: Several commercialized or commonly used methods,such as RNA Plant Reagent(Tiangen),Plant RNA Purification Reagent(Invitrogen),UNIQ column total RNA isolation kit(Sangon),SDS-LiCl method and TRIZOL Reagent(Invitrogen),etc.
方法:以灰绿藜叶片为材料,应用RNAPlant Reagent法(Tiangen)、Plant RNA purification Reagent法(Invitrogen)、UNIQ柱式总RNA试剂盒提取法(Sangon)、SDS-LiCl法、TRIZOL试剂法(Invitrogen)等进行总RNA的提取,并构建其全长cDNA文库和抑制消减文库。
2.
hispida in the four species,while the SDS-LiCl method failed to extract total RNA from F.
用Trizol法、SDS-LiCl法、改良CTAB法分别对对叶榕(Ficus hispida)、聚果榕(F。
2) SDS-LiCl improved method
SDS-LiCl改进法
1.
japonicus leaves as material, the effect of total RNA extraction using different methods of guanidine thiocyanate method,SDS-LiCl method and SDS-LiCl improved method was compared and the influence of the active carbon and ascorbic acid on the quality of RNA extraction was discussed.
[方法]以百脉根叶片为材料,比较异硫氰酸胍法、SDS-LiCl法及SDS-LiCl改进法提取总RNA的效果并探讨活性炭、抗坏血酸对RNA提取质量的影响。
3) LiCl method
LiCl法
1.
To look for rapid and efficient methods to isolate DNA,three algae(Porphyra yezoensis,Enteromorpha clathrata and Spirulina platensis)were as materials to extract DNA by three preparation methods,improved CTAB method,LiCl method and extraction kit.
选取三种不同海藻纲中的条斑紫菜、条浒苔、钝顶螺旋藻为材料,通过采用改良的CTAB法、LiCl法及试剂盒法抽提基因组DNA并进行比较,以找出适用于各种藻类DNA提取的快速有效方法。
4) SDS method
SDS法
1.
aconiti by using SDS method,CTAB method and the modified CTAB method resp.
[方法]以中药材附子药源植物的幼嫩叶片和块根为试材,分别采用SDS法、CTAB法和改良CTAB法从中药材附子药源植物中提取基因组DNA,比较不同方法的提取效果。
2.
This research reported a development SDS method for extraction cotton genomic DNA.
通过在目前常用的SDS法的DNA提取液中加入抗氧化剂(PVP40和β-巯基乙醇)排除棉酚等次生物质干扰,进行了棉花基因组DNA的提取方法研究,结果表明,改良后的SDS法能够解决棉花DNA提取质量不高且容易褐变等问题。
3.
To probe the adaptability of modified SDS method in apricot genomic DNA extraction and the optimal concentration, the genomic DNA samples of 6 apricot cultivars were isolated by 0.
为探讨改良SDS法是否适用于杏基因组DNA提取及提取液适宜浓度,以6个品种杏叶片为试材提取DNA,提取液设0。
5) SDS
SDS法
1.
We adopted the phenol-chloroform-isopentanol, CTAB and SDS methods to extract DNA from Sinononacula constricta , Meretix linnaeus,Chlamys nobilis, Haliotis diversicolor and other shellfishes.
以缢蛏、美人蛏、文蛤、扇贝和鲍鱼等几种经济贝类为研究对象,分别用苯酚/氯仿/异戊醇法,CTAB法,SDS法等不同方法提取其基因组DNA,寻找适宜于研究对象基因组DNA提取的方法,结果表明,3种方法均可以从目标材料中提取到DNA,但苯酚/氯仿/异戊醇法无论从数量和质量上都不理想,而SDS和CTAB可根据具体情况选择使用。
2.
The results showed that SDS-CTAB method was the best one,then SDS method,and CTAB method was not recommended.
以籽蒿(Artemisia sphaerocephala Krasch)种子为试验材料,采用SDS法、CTAB法及SDS-CTAB法进行基因组DNA提取。
3.
The tota1 DNA was extracted from the bean,horse bean and soybean with methods of high salt,SDS and CTAB.
以菜豆、蚕豆、黄豆的幼嫩叶片及其种子为材料,采用高盐低pH值法、SDS法和改良CTAB法3种不同的DNA提取方法提取其总DNA,用紫外分光光度法和琼脂糖凝胶电泳检测DNA质量。
6) LiCl precipitating method
LiCl沉淀法
1.
But capsules, especially capsule polysaccharides, will disturb DNA extracting largely, thus, LiCl precipitating method was introduced in this paper as a novel method and was examined by electrophoresis, spectrophotometer and amplification of 16S rRNA gene.
结合提取基因组DNA的CTAB法(hexadecyltrimethylammoniumbromide,十六烷基三甲基溴化铵)和用于提取海藻基因组DNA的LiCl法,设计了针对产生较多荚膜多糖细菌的DNA提取方法—LiCl沉淀法。
补充资料:licl
CAS:7447-41-8
分子式:ClLi
分子质量:42.39
中文名称:氯化锂;氯化锂(无水);氯化锂(结晶);氯化锂,无水
英文名称:Lithium chloride;Lithium chloride, anhydrous;chlorku litu;chlorure de lithium;licl;lithium chloride (licl);Lithiumchlorid
分子式:ClLi
分子质量:42.39
中文名称:氯化锂;氯化锂(无水);氯化锂(结晶);氯化锂,无水
英文名称:Lithium chloride;Lithium chloride, anhydrous;chlorku litu;chlorure de lithium;licl;lithium chloride (licl);Lithiumchlorid
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
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