1) Southern blotting analysis
Southern blotting杂交
2) Southern blot analysis
Southern blotting检测
3) Southern blot
Southern杂交
1.
Using RT-PCR/Southern blot to detect the change of Brn-4 mRNA expression in rat hippocampus after fimbria fornix transection;
RT-PCR/Southern杂交法检测穹窿海马伞切割大鼠海马Brn-4 mRNA的表达变化
2.
Methods 105 peripheral blood samples were collected from healthy individuals of different ages,ranged from 0 to 81 years old,and the terminal restriction fragment (TRF) length was measured by Southern blot analysis.
方法抽取西藏那曲地区0~81岁健康人外周血样本105例,其中男性53例,女性52例,采用Southern杂交法检测其端粒限制性片断平均长度。
3.
Methods Using RT PCR/Southern blot to detect the ALB mRNA in the peripheral blood from 29 patients with hepatocellular carcinoma (HCC) and 41 cases without HCC (8 cases of cirrhosis, 4 cases of acute hepatitis, 15 cases of chronic hepatitis, 2 cases of hepatic angioma, 2 cases of non HCC hepatic tumor and 10 normal voluteers).
方法 分离原发性肝癌患者以及肝硬化、急慢性肝炎、肝转移癌、肝脏良性肿瘤患者和健康志愿者的外周血单个核细胞 ,提取单个核细胞的总RNA ,进行RT PCR ,将RT PCR产物再进行Southern杂交。
4) Southern hybridization
Southern杂交
1.
Digested with restriction endonuclease, of chromosomal DNA was performed by Southern hybridization with pro.
对染色体 DNA进行限制性酶切 ,以第一类整合酶基因 int I1( DIG标记 )为探针 ,进行 Southern杂交。
2.
The result of Southern hybridization demonstrated that the differential fragments were true.
Southern杂交结果证明了差异片段的真实性。
3.
Digesting the constructs and making southern hybridization with the cDNA of rat 20 αHSD labeled with DIG,we revealed the 4.
0kb的DNA大片段 ,将此片段与载体质粒连接得到重组质粒 酶切重组质粒后电泳 ,再以地高辛标记的 2 0αHSD基因的cDNA为探针进行Southern杂交 实验获得了大片段的重组质
5) Southern blotting
Southern杂交
1.
Cloning,Southern blotting and RT-PCR analysis of a cDNA fragment encoding of 70 kDa heat shock cognate protein (Hsc70) from the oyster (Crassostrea ariakensis);
近江牡蛎Hsc70蛋白基因cDNA片段的克隆及Southern杂交和RT-PCR分析
2.
Methods\ Genomic DNA was digested with methylation sensitive enzymes and Southern blotting was used to analyse the methylation state of CDKN2/p16 gene in 89 cases of lung cancers.
方法 采用甲基化敏感性核酸内切酶酶切基因组DNA的方法,对89例肺癌的CDKN2p16基因进行了Southern杂交分析。
3.
PCR and Southern blotting further confirmed th.
并通过PCR和Southern杂交结果得到进一步验证。
6) southern blot hybridization
Southern杂交
1.
PCR and Southern blot hybridization assays showed that the plasmid f.
[方法 ]从人工构建的供体菌中诱导出带有标记的CTXΦ并转染至受体菌 ,用PCR扩增和Southern杂交等方法检测受体菌、转染子及其质粒的目的基因片段。
补充资料：blotting
分子式：
CAS号：
性质：方法是将层析或电泳分离后的DNA、RNA或蛋白质组分从支持物(如凝胶)上照原样转移到另一种支持物(如特种滤纸、薄膜)上，然后通过适当的探针进行鉴定，并可从中筛选出所需的单一组分。转移的方法可以依据毛细管作用，如DNA印迹转移(Southern blotting)、RNA印迹转移(Northern blotting)、蛋白质印迹(Western blot-ting)，亦可用电泳的方法，如电转移(electroblotting)
CAS号：
性质：方法是将层析或电泳分离后的DNA、RNA或蛋白质组分从支持物(如凝胶)上照原样转移到另一种支持物(如特种滤纸、薄膜)上，然后通过适当的探针进行鉴定，并可从中筛选出所需的单一组分。转移的方法可以依据毛细管作用，如DNA印迹转移(Southern blotting)、RNA印迹转移(Northern blotting)、蛋白质印迹(Western blot-ting)，亦可用电泳的方法，如电转移(electroblotting)
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
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