1) Southen and Northen blotting
Southern和Northern印迹杂交技术
2) Southern blot
Southern印迹杂交
1.
Southern blotting analysis of telomeric repetitive DNA sequences in Giardia canis;
犬贾第虫端粒重复序列的Southern印迹杂交分析
2.
Objective To confirm the integration of transduced genes in the genomic DNA of host cells by Southern blot analysis.
目的 应用Southern印迹杂交法 (Southernblot)证实转移基因整合至宿主细胞基因组DNA。
3) Northern blot
Northern印迹杂交
1.
Methods The probes corresponding to the genes were amplified by RT PCR from mouse kidney, and then Northern blotting was performed on mouse multiple tissues membrane.
方法RT-PCR方法扩增WNK1和WNK4基因片段作为探针,在小鼠多组织膜上进行Northern印迹杂交。
2.
Method The specific fragments of the two transcripts were amplified by RT-PCR and identified by Northern blot.
方法 RT PCR扩增两个转录本的特异性片段 ,Northern印迹杂交证实片段特异性后 ,将片段克隆入pGEM T载体中 ,体外转录同位素标记的正义和反义RNA探针 ,在小鼠肾脏组织石蜡切片上进行原位杂交检测。
3.
The gene expressions of matix metalloproteinase\|2(MMP\|2) and membrane type matrix metalloproteinase (MT\|MMP) and TIMP 1 were investigated by Northern blot,The gelatinase activity of MMP\|2 was exam.
方法 :以 0 5、2 0、4 0g kg等不同剂量抗纤复方灌胃大鼠制备的含药血清 ,作用HSC LI90细胞 4 8h ,应用Northern印迹杂交的方法 ,检测膜型基质金属蛋白酶、基质金属蛋白酶 2及其抑制因子 1(TIMP 1)基因的表达 ,并用酶图法检测基质金属蛋白酶 2的活性。
4) Northern blot analysis
Northern印迹杂交
1.
Northern blot analysis and immunocytochemistry techniques were performed to detect the expression of typeⅠ,Ⅲ procollagen mRNA and their relevant proteins in cultured rat pulmonary fibrobasts(PFbs) after FN treatment.
方法 用免疫组织化学方法观察实验性大鼠肺纤维化纤维连接蛋白 (FN)的动态变化 ;用Northern印迹杂交和免疫细胞化学方法分别观察FN对体外培养的大鼠肺成纤维细胞Ⅰ、Ⅲ型前胶原mRNA和蛋白表达的影响。
5) PCR-Southern blots analysis
PCR-Southern印迹杂交
1.
Through PCR analysis,the agarose gel electrophoresis result displayed that six of eight Kanamycin fastness seedlings had target genes;Through PCR-Southern blots analysis,the result showed that the positive rate was 50%,and further indicated that the target genes had been inserted into the genomes of Populus nigra×Populus deltoids "108" plants successfully.
通过PCR检测,凝胶电泳结果显示8个卡那霉素抗性芽中,6个存在目的基因;经PCR-Southern印迹杂交检测,阳性率为50%,进一步表明目的基因成功整合到欧美杨108号基因组内。
6) reverse Northern hybridization
反向Northern杂交技术
补充资料：印迹杂交
分子式：
CAS号：
性质：在基因操作中，把DNA或RNA、蛋白质等在薄膜滤器上先经浸润，固定后，于薄膜滤器上进行杂交，生成杂种分子。为基因操作中最常用的技术。常用的操作方法是：将用凝胶电泳分离出来的DNA或RNA、蛋白质等，在保持电泳图形的条件下，使其移至薄膜滤器上，再用事先准备好的检测样品(探针，经标记的某种基因等)进行杂交。以显示这些DNA、RNA、蛋白质等。从凝胶上转移DNA的技术称萨瑟恩(Southern)印迹技术。一般把转移RNA称为诺森(Northern)印迹技术，而把转移蛋白质称为韦斯顿(Western)印迹技术，当利用凝胶进行抗原抗体反应，再进行印迹时，称做伊斯特尔(Eastern)印迹技术。
CAS号：
性质：在基因操作中，把DNA或RNA、蛋白质等在薄膜滤器上先经浸润，固定后，于薄膜滤器上进行杂交，生成杂种分子。为基因操作中最常用的技术。常用的操作方法是：将用凝胶电泳分离出来的DNA或RNA、蛋白质等，在保持电泳图形的条件下，使其移至薄膜滤器上，再用事先准备好的检测样品(探针，经标记的某种基因等)进行杂交。以显示这些DNA、RNA、蛋白质等。从凝胶上转移DNA的技术称萨瑟恩(Southern)印迹技术。一般把转移RNA称为诺森(Northern)印迹技术，而把转移蛋白质称为韦斯顿(Western)印迹技术，当利用凝胶进行抗原抗体反应，再进行印迹时，称做伊斯特尔(Eastern)印迹技术。
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参考词条