1) RT-PCR-Southern blot
RT-PCR-Southern杂交
2) PCR-Southern blotting
PCR-Southern杂交
1.
The results showed that two special fragments of about 460 bp and 690 bp were obtained,and PCR-Southern blotting about the.
用抗根结线虫基因设计的特异引物进行扩增最终获得了1条大约460 bp大小的特异片段;用抗胞囊线虫基因设计的特异引物进行扩增最终获得了1条大约690 bp大小的特异片段,进一步进行了PCR-Southern杂交,确认了该2条特异引物的真实性。
3) PCR-Southern hybridization
PCR-Southern分子杂交
4) PCR-Southern blots analysis
PCR-Southern印迹杂交
1.
Through PCR analysis,the agarose gel electrophoresis result displayed that six of eight Kanamycin fastness seedlings had target genes;Through PCR-Southern blots analysis,the result showed that the positive rate was 50%,and further indicated that the target genes had been inserted into the genomes of Populus nigra×Populus deltoids "108" plants successfully.
通过PCR检测,凝胶电泳结果显示8个卡那霉素抗性芽中,6个存在目的基因;经PCR-Southern印迹杂交检测,阳性率为50%,进一步表明目的基因成功整合到欧美杨108号基因组内。
5) PCR in situ hybridization
RT-PCR原位杂交
6) HC-RT-F-PCR
杂交诱捕-RT-PCR-ELISA
补充资料:immune PCR
分子式:
CAS号:
性质: 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上,从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上,进而证明有抗原存在。目前最为敏感的检测方法,理论上可测得一个抗原分子的存在。
CAS号:
性质: 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上,从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上,进而证明有抗原存在。目前最为敏感的检测方法,理论上可测得一个抗原分子的存在。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条