1) PCR-Southern blots analysis
PCR-Southern印迹杂交
1.
Through PCR analysis,the agarose gel electrophoresis result displayed that six of eight Kanamycin fastness seedlings had target genes;Through PCR-Southern blots analysis,the result showed that the positive rate was 50%,and further indicated that the target genes had been inserted into the genomes of Populus nigra×Populus deltoids "108" plants successfully.
通过PCR检测,凝胶电泳结果显示8个卡那霉素抗性芽中,6个存在目的基因;经PCR-Southern印迹杂交检测,阳性率为50%,进一步表明目的基因成功整合到欧美杨108号基因组内。
2) Southern blot
Southern印迹杂交
1.
Southern blotting analysis of telomeric repetitive DNA sequences in Giardia canis;
犬贾第虫端粒重复序列的Southern印迹杂交分析
2.
Objective To confirm the integration of transduced genes in the genomic DNA of host cells by Southern blot analysis.
目的 应用Southern印迹杂交法 (Southernblot)证实转移基因整合至宿主细胞基因组DNA。
3) PCR-Southern blotting
PCR-Southern杂交
1.
The results showed that two special fragments of about 460 bp and 690 bp were obtained,and PCR-Southern blotting about the.
用抗根结线虫基因设计的特异引物进行扩增最终获得了1条大约460 bp大小的特异片段;用抗胞囊线虫基因设计的特异引物进行扩增最终获得了1条大约690 bp大小的特异片段,进一步进行了PCR-Southern杂交,确认了该2条特异引物的真实性。
4) RT-PCR-Southern blot
RT-PCR-Southern杂交
5) Southen and Northen blotting
Southern和Northern印迹杂交技术
6) PCR-Southern hybridization
PCR-Southern分子杂交
补充资料：PC
聚碳酸酯(双酚A型)化学名称2，2-双(4-羟基苯基)丙烷聚碳酸酯，是由酯交换法和光气化法制得。平均分子量3.57×104。无定形透明颗粒．无味、天臭、无毒。相对密度1．20-1.43。没有明显熔点，220-230℃呈熔融状态。透光率高，吸水性低，耐冲击，韧性好．蠕变小，制品尺寸稳定。耐热性和耐寒性较好．玻璃化温度149-150℃．热变形温度135℃(1．82MPa)、分解温度＞310 ℃，脆性温度－l00 ℃，使用温度-100～120 ℃。耐稀酸、耐油、不耐碱。溶于二氯甲烷、二氯乙烷、氯仿、三氯乙烯、四氯乙烷、四氢呋喃、三甲酚、磷酸三甲酯等。疲劳强度较低，容易产生应力开裂。具有优良的高温电性能，具有耐燃自熄性。拉伸强度 ＞60MPa, 伸长率＞60％，弯曲强度＞95MPa。
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参考词条