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1)  Southern blot
Southern印迹杂交
1.
Southern blotting analysis of telomeric repetitive DNA sequences in Giardia canis;
犬贾第虫端粒重复序列的Southern印迹杂交分析
2.
Objective To confirm the integration of transduced genes in the genomic DNA of host cells by Southern blot analysis.
目的 应用Southern印迹杂交法 (Southernblot)证实转移基因整合至宿主细胞基因组DNA。
2)  PCR-Southern blots analysis
PCR-Southern印迹杂交
1.
Through PCR analysis,the agarose gel electrophoresis result displayed that six of eight Kanamycin fastness seedlings had target genes;Through PCR-Southern blots analysis,the result showed that the positive rate was 50%,and further indicated that the target genes had been inserted into the genomes of Populus nigra×Populus deltoids "108" plants successfully.
通过PCR检测,凝胶电泳结果显示8个卡那霉素抗性芽中,6个存在目的基因;经PCR-Southern印迹杂交检测,阳性率为50%,进一步表明目的基因成功整合到欧美杨108号基因组内。
3)  Southen and Northen blotting
Southern和Northern印迹杂交技术
4)  Southern blotting
Southern印迹
1.
Methods PFGE(pulsed field gradient gel electrophoresis)combined with Southern blotting hybridization was used to determine the sizes of DMs in human ovarian cancer cells UACC-1598 and MTX-resistant cell derived from mouse fibroblasts 3T3R500.
方法应用PFGE(Pulsed field gradient gel electrophoresis)与Southern印迹杂交技术,对人卵巢癌细胞UACC-1598及氨甲蝶呤(Methotrexate,MTX)抗性的小鼠胚胎成纤维细胞中DMs的大小进行检测。
2.
Methods: Telomere length was examined by Southern blotting by using labelled oligonucleotide(TTAGGG)3 probe with digoxin.
方法:以地高辛标记寡核苷酸(TTAGGG)3为探针,对基因组DNA进行Southern印迹检测,经CDP-Star显色后与DNA标准分子量比较,利用图像分析系统计算端粒长度。
5)  Southern blot
Southern印迹
1.
Methods The length of D4Z4 repeats on chromosome 4q35 in 191 normal individuals in Shanghai was determined by pulsed-field gel electrophoresis and Southern blotting after double digestion with EcoRⅠ and BlnⅠ.
方法191名正常上海人的基因组DNA经EcoRⅠ/B1nⅠ双酶水解后,应用脉冲场凝胶电泳及Southern印迹测定其染色体4q35位点的D4Z4片段长度,并对短的D4Z4片段用KpnⅠ酶进行部分酶切以计数其D4Z4串联重复序列数。
2.
Methods Densitometry of the bands on Southern blot, polymerase.
方法 用 Southern印迹方法检测 7例 CPEO和 4例 KSS患者的肌肉组织 mt DNA,并进一步用聚合酶链反应产物直接测序来明确缺失的具体范围 ;用聚合酶链反应 -限制性内切酶分析法检测有无 mt D-NA A32 4 3G点突变。
3.
A simple,rapid,sensitive,low-cost and reproducible protocol of Southern blot analysis was developed based on a comprehensive analysis of the previous reports.
在综合前人方法的基础上,改良了一种简捷、灵敏、廉价、效果可靠的Southern印迹杂交方案,使用0。
6)  Southern blot
Southern印迹法
补充资料:印迹杂交
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性质:在基因操作中,把DNA或RNA、蛋白质等在薄膜滤器上先经浸润,固定后,于薄膜滤器上进行杂交,生成杂种分子。为基因操作中最常用的技术。常用的操作方法是:将用凝胶电泳分离出来的DNA或RNA、蛋白质等,在保持电泳图形的条件下,使其移至薄膜滤器上,再用事先准备好的检测样品(探针,经标记的某种基因等)进行杂交。以显示这些DNA、RNA、蛋白质等。从凝胶上转移DNA的技术称萨瑟恩(Southern)印迹技术。一般把转移RNA称为诺森(Northern)印迹技术,而把转移蛋白质称为韦斯顿(Western)印迹技术,当利用凝胶进行抗原抗体反应,再进行印迹时,称做伊斯特尔(Eastern)印迹技术。

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