1) Southern blotting hybridization
Southern转膜杂交
2) Southern blot
Southern杂交
1.
Using RT-PCR/Southern blot to detect the change of Brn-4 mRNA expression in rat hippocampus after fimbria fornix transection;
RT-PCR/Southern杂交法检测穹窿海马伞切割大鼠海马Brn-4 mRNA的表达变化
2.
Methods 105 peripheral blood samples were collected from healthy individuals of different ages,ranged from 0 to 81 years old,and the terminal restriction fragment (TRF) length was measured by Southern blot analysis.
方法抽取西藏那曲地区0~81岁健康人外周血样本105例,其中男性53例,女性52例,采用Southern杂交法检测其端粒限制性片断平均长度。
3.
Methods Using RT PCR/Southern blot to detect the ALB mRNA in the peripheral blood from 29 patients with hepatocellular carcinoma (HCC) and 41 cases without HCC (8 cases of cirrhosis, 4 cases of acute hepatitis, 15 cases of chronic hepatitis, 2 cases of hepatic angioma, 2 cases of non HCC hepatic tumor and 10 normal voluteers).
方法 分离原发性肝癌患者以及肝硬化、急慢性肝炎、肝转移癌、肝脏良性肿瘤患者和健康志愿者的外周血单个核细胞 ,提取单个核细胞的总RNA ,进行RT PCR ,将RT PCR产物再进行Southern杂交。
3) Southern hybridization
Southern杂交
1.
Digested with restriction endonuclease, of chromosomal DNA was performed by Southern hybridization with pro.
对染色体 DNA进行限制性酶切 ,以第一类整合酶基因 int I1( DIG标记 )为探针 ,进行 Southern杂交。
2.
The result of Southern hybridization demonstrated that the differential fragments were true.
Southern杂交结果证明了差异片段的真实性。
3.
Digesting the constructs and making southern hybridization with the cDNA of rat 20 αHSD labeled with DIG,we revealed the 4.
0kb的DNA大片段 ,将此片段与载体质粒连接得到重组质粒 酶切重组质粒后电泳 ,再以地高辛标记的 2 0αHSD基因的cDNA为探针进行Southern杂交 实验获得了大片段的重组质
4) Southern blotting
Southern杂交
1.
Cloning,Southern blotting and RT-PCR analysis of a cDNA fragment encoding of 70 kDa heat shock cognate protein (Hsc70) from the oyster (Crassostrea ariakensis);
近江牡蛎Hsc70蛋白基因cDNA片段的克隆及Southern杂交和RT-PCR分析
2.
Methods\ Genomic DNA was digested with methylation sensitive enzymes and Southern blotting was used to analyse the methylation state of CDKN2/p16 gene in 89 cases of lung cancers.
方法 采用甲基化敏感性核酸内切酶酶切基因组DNA的方法,对89例肺癌的CDKN2p16基因进行了Southern杂交分析。
3.
PCR and Southern blotting further confirmed th.
并通过PCR和Southern杂交结果得到进一步验证。
5) southern blot hybridization
Southern杂交
1.
PCR and Southern blot hybridization assays showed that the plasmid f.
[方法 ]从人工构建的供体菌中诱导出带有标记的CTXΦ并转染至受体菌 ,用PCR扩增和Southern杂交等方法检测受体菌、转染子及其质粒的目的基因片段。
6) Southern blot hybridization
Southern Blot杂交
补充资料：世间转法转智转无尽
【世间转法转智转无尽】
世间转者，谓展转摄前众生界、世界、虚空界也。法转者，谓展转摄前法界、涅槃界、佛出现界也。智转者，谓展转摄前如来智界，心所缘佛智所入境界也。盖此三转，皆言无尽者，以世法智之三种，展转含摄，无有穷尽，是名世间转、法转、智转无尽。
世间转者，谓展转摄前众生界、世界、虚空界也。法转者，谓展转摄前法界、涅槃界、佛出现界也。智转者，谓展转摄前如来智界，心所缘佛智所入境界也。盖此三转，皆言无尽者，以世法智之三种，展转含摄，无有穷尽，是名世间转、法转、智转无尽。
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