2) Short hairpin RNA
短发夹RNA
1.
Construction and identification of a vector expressing short hairpin RNA targeting annexinⅡgene;
膜联蛋白Ⅱ基因短发夹RNA表达载体的构建和鉴定
2.
The effect of short hairpin RNA on hTERT expression;
短发夹RNA对人端粒酶催化亚基表达的影响
3.
Objective To clone shRNA (short hairpin RNA) recombinant plasmid targeting on COX-2 gene and analyze the nucleic acid sequence of the recombinant plasmid.
目的利用RNA干扰技术,以COX-2为靶基因,构建靶向COX-2基因的短发夹RNA(shRNA)重组表达质粒并进行鉴定分析。
3) shRNA
短发夹RNA
1.
Inhibition of ICAM-1 gene expression in liver cancer cells by shRNA;
短发夹RNA对肝癌细胞细胞间粘附分子-1基因表达的影响
2.
Objectives To investigate nano-carrier G9 PAMAM mediated plasmid containing CTGF-shRNA (pCTGF-shRNA) transfection on connective tissue growth factor (CTGF) expression in mouse peritoneal mesothelial cells (MPMCs).
目的研究G9PAMAM纳米载体介导的结缔组织生长因子(CTGF)短发夹RNA(shRNA)质粒(pCTGF-shRNA)对小鼠腹膜间皮细胞CTGF表达的影响。
3.
The annealed oligonucleotides were inserted into the linearized pSIREN-RetroQ vector to construct the recombinant shRNA plasmid(pSIREN-VEGF-C) and then transformed into E.
方法:根据GenBank中VEGF-C序列,设计、合成靶向VEGF-C基因、编码短发夹RNA的两条寡核苷酸序列,退火后用T4连接酶与线性化的pSIREN载体连接,转化感受态大肠杆菌,筛选阳性克隆,抽提质粒,获得重组pSIREN-VEGF-C质粒;BglⅡ和EcoRⅠ双酶切、测序鉴定。
4) short hairpin RNA(shRNA)
短发夹RNA
1.
Objective: To transfect a recombinant short hairpin RNA(shRNA)expression vector targeting Livin gene isoform(BIRC71,BIRC72)into the cervical cancer cell line(Hela cell),in an attempt to observe RNAi-mediated silen- cing on Livin gene and the induction of Hela apoptosis.
目的:应用构建成功的Livin异构体(BIRC71,BIRC72)短发夹RNA(short hairpin HNA,shRNA)真核表达载体转染Hela细胞,探讨其对Livin基因的沉默效应及诱导Hela细胞凋亡的作用。
5) Short-hairpin RNA
短发夹RNA
1.
Objective: To construct short-hairpin RNA expressing vectors aimed at nuclear receptor-related factor 1 (Nurrl) and study the function of Nurrl with RNA interference technology.
目的:构建针对小鼠核受体相关因子1(Nurrl)的短发夹RNA(shRNA)表达载体,为进一步体内外研究Nurrl基因的功能奠定基础。
2.
Objective To construct effective short-hairpin RNA (shRNA) expression vector encoding shRNA targeting LRIG2 gene, and to screen the stably transfected cell clone.
目的构建针对富含亮氨酸重复序列免疫球蛋白样蛋白2(LRIG2)基因的短发夹RNA(shRNA)表达载体,建立稳定转染的细胞株,观察其对目的基因表达的影响。
6) hPARP1
短发夹-RNA
补充资料：发夹结构
分子式：
CAS号：
性质：即当具有回文结构的DNA变性后，倒转原来呈现回文结构的重复DNA序列之间可以形成发夹结构或称十字形结构(cruciform structure)，（图暂缺）。除了转运氨基酸的tRNA的二级结构模型呈现发夹结构外，真核细胞染色体DNA结构上也有发夹结构出现，多位学者已用电镜在许多真核细胞DNA中观察到长度为350～1200碱基对(bp)的发夹形结构。DNA中回文结构尚不十分清楚，推测与DNA-蛋白质之间的识别有关。
CAS号：
性质：即当具有回文结构的DNA变性后，倒转原来呈现回文结构的重复DNA序列之间可以形成发夹结构或称十字形结构(cruciform structure)，（图暂缺）。除了转运氨基酸的tRNA的二级结构模型呈现发夹结构外，真核细胞染色体DNA结构上也有发夹结构出现，多位学者已用电镜在许多真核细胞DNA中观察到长度为350～1200碱基对(bp)的发夹形结构。DNA中回文结构尚不十分清楚，推测与DNA-蛋白质之间的识别有关。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条