2) small hairpin RNA
小发夹结构RNA
1.
Objective To construct eukaryotic expression plasmid of small hairpin RNA(shRNA) targeting PCNA,express plasmid in HeLa cells and investigate the inhibitory effect of shRNA targeting PCNA on proliferation and biological features of HeLa cells in vitro.
目的构建增殖细胞核抗原(PCNA)的小发夹结构RNA(shRNA)的真核表达载体并在人HeLa细胞表达,同时观察PCNA shRNA对HeLa细胞体外增殖及生物学特性的影响。
2.
Inhibition of HeLa Cells by Small Hairpin RNA of PCNA;
目的:构建增殖细胞抗原(PCNA)的小发夹结构RNA(shRNA)的真核表达载体并在HeLa细胞表达,同时观察PCNA的shRNA对人HeLa细胞株体外增殖的影响及生物学特性的改变。
4) miR-30-based shRNA
基于miR30的发夹结构RNA
5) Short hairpin RNA
短发夹RNA
1.
Construction and identification of a vector expressing short hairpin RNA targeting annexinⅡgene;
膜联蛋白Ⅱ基因短发夹RNA表达载体的构建和鉴定
2.
The effect of short hairpin RNA on hTERT expression;
短发夹RNA对人端粒酶催化亚基表达的影响
3.
Objective To clone shRNA (short hairpin RNA) recombinant plasmid targeting on COX-2 gene and analyze the nucleic acid sequence of the recombinant plasmid.
目的利用RNA干扰技术,以COX-2为靶基因,构建靶向COX-2基因的短发夹RNA(shRNA)重组表达质粒并进行鉴定分析。
6) shRNA
短发夹RNA
1.
Inhibition of ICAM-1 gene expression in liver cancer cells by shRNA;
短发夹RNA对肝癌细胞细胞间粘附分子-1基因表达的影响
2.
Objectives To investigate nano-carrier G9 PAMAM mediated plasmid containing CTGF-shRNA (pCTGF-shRNA) transfection on connective tissue growth factor (CTGF) expression in mouse peritoneal mesothelial cells (MPMCs).
目的研究G9PAMAM纳米载体介导的结缔组织生长因子(CTGF)短发夹RNA(shRNA)质粒(pCTGF-shRNA)对小鼠腹膜间皮细胞CTGF表达的影响。
3.
The annealed oligonucleotides were inserted into the linearized pSIREN-RetroQ vector to construct the recombinant shRNA plasmid(pSIREN-VEGF-C) and then transformed into E.
方法:根据GenBank中VEGF-C序列,设计、合成靶向VEGF-C基因、编码短发夹RNA的两条寡核苷酸序列,退火后用T4连接酶与线性化的pSIREN载体连接,转化感受态大肠杆菌,筛选阳性克隆,抽提质粒,获得重组pSIREN-VEGF-C质粒;BglⅡ和EcoRⅠ双酶切、测序鉴定。
补充资料：RNA
见核糖核酸。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条