1) short hairpin RNA
短发夹环RNA
1.
Construction and identification of plasmid containing short hairpin RNA of angiotensin Ⅱ type 1 receptor of rats;
鼠血管紧张素Ⅱ受体1短发夹环RNA质粒的构建与鉴定
2.
Objective: To generate eukaryotic expression vector of short hairpin RNA(shRNA) specific for Y-box binding protein-1(YB-1).
目的:构建Y-盒结合蛋白1(Y-box binding protein-1,YB-1)特异性短发夹环RNA(shRNA)真核表达载体。
3.
The present study was designed to investigate whether the plasmid containing the short hairpin RNA(shRNA) of angiotensin Ⅱ type 1 receptor(AT1R) can inhibit the hyperplasia of VSMCs in rat.
本研究旨在探讨血管紧张素Ⅱ 1型受体 (AT1R)的短发夹环RNA质粒 (pAT1R shRNA)对大鼠血管平滑肌细胞 (VSMC)增殖的影响。
2) Short hairpin RNA
短发夹RNA
1.
Construction and identification of a vector expressing short hairpin RNA targeting annexinⅡgene;
膜联蛋白Ⅱ基因短发夹RNA表达载体的构建和鉴定
2.
The effect of short hairpin RNA on hTERT expression;
短发夹RNA对人端粒酶催化亚基表达的影响
3.
Objective To clone shRNA (short hairpin RNA) recombinant plasmid targeting on COX-2 gene and analyze the nucleic acid sequence of the recombinant plasmid.
目的利用RNA干扰技术,以COX-2为靶基因,构建靶向COX-2基因的短发夹RNA(shRNA)重组表达质粒并进行鉴定分析。
3) shRNA
短发夹RNA
1.
Inhibition of ICAM-1 gene expression in liver cancer cells by shRNA;
短发夹RNA对肝癌细胞细胞间粘附分子-1基因表达的影响
2.
Objectives To investigate nano-carrier G9 PAMAM mediated plasmid containing CTGF-shRNA (pCTGF-shRNA) transfection on connective tissue growth factor (CTGF) expression in mouse peritoneal mesothelial cells (MPMCs).
目的研究G9PAMAM纳米载体介导的结缔组织生长因子(CTGF)短发夹RNA(shRNA)质粒(pCTGF-shRNA)对小鼠腹膜间皮细胞CTGF表达的影响。
3.
The annealed oligonucleotides were inserted into the linearized pSIREN-RetroQ vector to construct the recombinant shRNA plasmid(pSIREN-VEGF-C) and then transformed into E.
方法:根据GenBank中VEGF-C序列,设计、合成靶向VEGF-C基因、编码短发夹RNA的两条寡核苷酸序列,退火后用T4连接酶与线性化的pSIREN载体连接,转化感受态大肠杆菌,筛选阳性克隆,抽提质粒,获得重组pSIREN-VEGF-C质粒;BglⅡ和EcoRⅠ双酶切、测序鉴定。
4) short hairpin RNA(shRNA)
短发夹RNA
1.
Objective: To transfect a recombinant short hairpin RNA(shRNA)expression vector targeting Livin gene isoform(BIRC71,BIRC72)into the cervical cancer cell line(Hela cell),in an attempt to observe RNAi-mediated silen- cing on Livin gene and the induction of Hela apoptosis.
目的:应用构建成功的Livin异构体(BIRC71,BIRC72)短发夹RNA(short hairpin HNA,shRNA)真核表达载体转染Hela细胞,探讨其对Livin基因的沉默效应及诱导Hela细胞凋亡的作用。
5) Short-hairpin RNA
短发夹RNA
1.
Objective: To construct short-hairpin RNA expressing vectors aimed at nuclear receptor-related factor 1 (Nurrl) and study the function of Nurrl with RNA interference technology.
目的:构建针对小鼠核受体相关因子1(Nurrl)的短发夹RNA(shRNA)表达载体,为进一步体内外研究Nurrl基因的功能奠定基础。
2.
Objective To construct effective short-hairpin RNA (shRNA) expression vector encoding shRNA targeting LRIG2 gene, and to screen the stably transfected cell clone.
目的构建针对富含亮氨酸重复序列免疫球蛋白样蛋白2(LRIG2)基因的短发夹RNA(shRNA)表达载体,建立稳定转染的细胞株,观察其对目的基因表达的影响。
6) hPARP1
短发夹-RNA
补充资料：发夹环
分子式：
CAS号：
性质：通常是对核酸中具有转运氨基酸作用的tRNA分子二级结构(模型)的一种形象的比喻。在tRNA一级结构上几十个核苷酸中都可以找出一些能配对的区域而形成局部的双链段形似发夹的直端，然而中间不能配对的碱基便鼓出而称为发夹的环状部件，故称发夹环。整个tRNA分子形似发夹，称为发夹结构(hairpin structure)。在酵母菌酪氨酸-tRNA的二级结构模型中，92个核苷酸的单链在形成二级结构是呈现发夹结构或三叶草形。同理，也常把tRNA二级结构比喻为三叶草形或柄叶形(stem loop)。双螺旋区构成了叶柄，突环区为三叶草的三片小叶，故又称柄叶结构或三叶草结构(cloverleaf pattern)。
CAS号：
性质：通常是对核酸中具有转运氨基酸作用的tRNA分子二级结构(模型)的一种形象的比喻。在tRNA一级结构上几十个核苷酸中都可以找出一些能配对的区域而形成局部的双链段形似发夹的直端，然而中间不能配对的碱基便鼓出而称为发夹的环状部件，故称发夹环。整个tRNA分子形似发夹，称为发夹结构(hairpin structure)。在酵母菌酪氨酸-tRNA的二级结构模型中，92个核苷酸的单链在形成二级结构是呈现发夹结构或三叶草形。同理，也常把tRNA二级结构比喻为三叶草形或柄叶形(stem loop)。双螺旋区构成了叶柄，突环区为三叶草的三片小叶，故又称柄叶结构或三叶草结构(cloverleaf pattern)。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条