1) small hairpin RNA
小发夹结构RNA
1.
Objective To construct eukaryotic expression plasmid of small hairpin RNA(shRNA) targeting PCNA,express plasmid in HeLa cells and investigate the inhibitory effect of shRNA targeting PCNA on proliferation and biological features of HeLa cells in vitro.
目的构建增殖细胞核抗原(PCNA)的小发夹结构RNA(shRNA)的真核表达载体并在人HeLa细胞表达,同时观察PCNA shRNA对HeLa细胞体外增殖及生物学特性的影响。
2.
Inhibition of HeLa Cells by Small Hairpin RNA of PCNA;
目的:构建增殖细胞抗原(PCNA)的小发夹结构RNA(shRNA)的真核表达载体并在HeLa细胞表达,同时观察PCNA的shRNA对人HeLa细胞株体外增殖的影响及生物学特性的改变。
4) small hairpin RNA
小发夹RNA
1.
Now there are four main kinds of RNA interference libraries with different construction approaches and molecular form,chemically synthesized small interference RNA(siRNA) libraries,chemically synthesized small hairpin RNA(shRNA) libraries,enzymatically prepared siRNA libraries and enzymatically prepared shRNA libraries.
不同种类的RNA干扰库已经被用于基因功能的研究之中,根据其构建方式和分子形式的不同,可将RNA干扰库分为4种类型,即化学合成的小干扰RNA(siRNA)库、化学合成的小发夹RNA(shRNA)表达库、酶切法构建的siRNA库和酶切法构建的shRNA表达库。
2.
[Methods] A shRNA expressed vector that expresses the specific small hairpin RNA targeting TRAP mRNA was constructed and transfected into SGC-7901 cells, and the stably expressing shRNA cells were selected by G418 and continuously cultured in half the antibiotic concentration for 3 months.
目的探讨稳定转染靶向端粒酶调节相关基因TRAP的小发夹RNA对胃癌细胞株SGC-7901的长效影响。
3.
[Methods] Plasmid vectors expressing small hairpin RNA (shRNA) targeting at cyclin D1 gene were constructed and transfected into K562 cells mediated by chitosan.
[方法]体外构建靶向cyclin D1基因的小发夹RNA(shRNA)表达质粒,通过壳聚糖介导转染K562细胞,Western blot分析检测转染前后cyclin D1蛋白表达变化;集落形成实验检测细胞增殖能力;流式细胞仪检测细胞周期分布及凋亡情况。
5) shRNA
小发夹RNA
1.
Construction Expressing Vector of shRNA Inhibiting Cyclin D1 Gene Expression by RNA Interfering;
抑制cyclin D1基因小发夹RNA(shRNA)表达质粒的构建
2.
[Methods] The 60nt nucleiotide sequence encoded targeting EBNA2 shRNA was cloned into a retroviral vector pSUPER.
[方法]用DNA重组技术将60nt能转录产生靶向EBNA2小发夹RNA(smallhairpin RNA,shRNA)的寡核苷酸序列定向插入逆转录病毒载体pSUPER。
3.
Objective:To construct and identify the small hairpin RNA(shRNA) expression vector for Smad 4/DPC 4 gene.
目的:构建Smad4基因小发夹RNA(shRNA)表达载体。
6) Short hairpin RNA
小发夹RNA
1.
RNAi with short hairpin RNA (shRNA) has been successfully utilized in a number of recent studies in cultured mammalian cells to reduce the expression of specific cellular and viral genes.
在哺乳动物细胞里,基于小发夹RNA(shRNA)的RNA干扰技术可以成功下调细胞或者病毒基因的表达。
2.
This study was purposed to investigate the inhibitory effect of short hairpin RNA (shRNA) on expression of vascular endothelial growth factor (VEGF) receptor flt-1 gene in leukemia cells line K562,and to explore the influence of shRNA invasive ability on leukemia cells and its mechanism.
本研究观察小发夹RNA对白血病细胞血管内皮生长因子受体flt-1基因表达的抑制作用并探讨其对白血病细胞侵袭能力的影响及作用机制。
补充资料：发夹结构
分子式：
CAS号：
性质：即当具有回文结构的DNA变性后，倒转原来呈现回文结构的重复DNA序列之间可以形成发夹结构或称十字形结构(cruciform structure)，（图暂缺）。除了转运氨基酸的tRNA的二级结构模型呈现发夹结构外，真核细胞染色体DNA结构上也有发夹结构出现，多位学者已用电镜在许多真核细胞DNA中观察到长度为350～1200碱基对(bp)的发夹形结构。DNA中回文结构尚不十分清楚，推测与DNA-蛋白质之间的识别有关。
CAS号：
性质：即当具有回文结构的DNA变性后，倒转原来呈现回文结构的重复DNA序列之间可以形成发夹结构或称十字形结构(cruciform structure)，（图暂缺）。除了转运氨基酸的tRNA的二级结构模型呈现发夹结构外，真核细胞染色体DNA结构上也有发夹结构出现，多位学者已用电镜在许多真核细胞DNA中观察到长度为350～1200碱基对(bp)的发夹形结构。DNA中回文结构尚不十分清楚，推测与DNA-蛋白质之间的识别有关。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条