1) RT-nested-PCR
反转录套式PCR
1.
Isolated from some sick pigeons,a virus named PSH and proved to be ty pical coronavirus by injection of SPF chicken embryos,observing by electronscope ,animal regress test and RT-nested-PCR test.
从有呼吸道症状、有胰脏充出血、肺脏肉样病变的肉鸽中,通过鸡胚接种、电镜观察和反转录套式PCR分析,证实分离的病毒为冠状病毒;经过动物试验,鸡传染性支气管炎标准毒株I(BVM41)不引起试验鸽感染,而本次分离株对试验鸽具有一定的致病性,表明该分离株与IBV有较大差异,暂命名为鸽冠状病毒P(SH)。
2) RT-nested PCR
逆转录套式PCR
1.
Development of RT-nested PCR for detection of antisense RNA recombinant retrovirus;
反义RNA重组逆转录病毒逆转录套式PCR检测方法的建立
2.
Detection of Newcastle Disease Virus by RT-nested PCR;
应用逆转录套式PCR检测新城疫病毒核酸
3) RT-nested PCR
反转录巢式PCR
4) RT-PCR
反转录PCR
1.
Methods: The expression of 5-HT receptor subtype mRNAs was detected in the lumbar dorsal root ganglion (DRG) by reverse transcription-polymerase chain reaction(RT-PCR) following unilateral injection of formalin into the plantar surface of rat hind paw.
方法:用反转录PCR技术观察大鼠单侧足底皮下注射福尔马林致痛后背根节内5-HT1~7受体亚型mRNAs的表达变化。
2.
?Methods Using the total RNA extracted from fresh rat hippocampus as template,the cDNA of PDZ1 was amplified by RT-PCR;the cDNA obtained was subsequently cloned into the shuttle vector pAdTrack-CMV(CLONTECH),and then,the sequence of the product was determined.
方法以异硫酸氢胍-酚-氯仿一步法提取的总RNA为模板,采用反转录PCR(RT-PCR)法获得PDZ1的cDNA;与腺病毒穿梭载体用T4DNA连接酶连接;连接产物转化大肠杆菌JM109进行筛选、序列测定。
3.
Methods:BMP7 gene was cloned from the HEK293 cells with RT-PCR and nested-PCR skills,and after being repaired,the whole cDNA of BMP7 was obtained.
方法 :通过反转录PCR技术和巢式PCR技术从人类胚胎肾 2 93细胞中克隆BMP7基因 ,应用互补拼接的方法对其进行修复获得全长cDNA ,将其与T载体进行连接得到BMP7克隆质粒。
5) RT-PCR
反转录-PCR
1.
0kb DNA fragment was obtained by RT-PCR fr.
本研究以黑曲霉M-1为出发菌株，设计特异的引物，采用反转录-PCR技术直接从黑曲霉总RNA中反转录并扩增出约3。
6) RT PCR
反转录PCR
1.
cDNA fragment encoding human P34H was amplified by RT PCR using specific primers, and then was cloned into pGEM T vector.
方法 :提取人附睾体部总RNA ,并以此为模板 ,进行反转录PCR获得编码P34H蛋白的基因片段。
2.
Methods:Tumor tissues and adjacent normal tissues in 41 cases of esophageal carcinoma were studied by using the reverse transcriptase polymerase chain reaction(RT PCR).
方法 :用反转录PCR(RT PCR)法检测 41例食管癌患者的癌组织、癌旁组织MMP 2mRNA的表达。
补充资料：反转录PCR
分子式：
CAS号：
性质：以RNA为起始对象，经反转录酶作用合成cDNA后，再用DNA引物进行DNA扩增，两个反向连续进行，叫作反转录PCR。
CAS号：
性质：以RNA为起始对象，经反转录酶作用合成cDNA后，再用DNA引物进行DNA扩增，两个反向连续进行，叫作反转录PCR。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条