原位反转录PCR,in situ RT-PCR,音标,读音,翻译,英文例句,英语词典

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1) in situ RT-PCR 点击朗读

原位反转录PCR

The principle, key factors and steps of in situ RT-PCR including tissues or cells fixation, primer and probe selections, protease or DNase digestions, false positive and false negative results were summarized to emphasize the application of in situ RT-PCR to the actual detections.

针对已有资料中对于以DNA为靶基因序列的原位 PCR介绍较多,而对于原位反转录PCR的介绍很少,但在实际研究工作中有很多检测的靶基因是 RNA(病毒的或基因组的 RNA)的实际情况,就原位反转录PCR技术的基本原理、影响试验结果的关键因素和步骤,如组织细胞的固定、引物和探针的选择、蛋白酶及DNA酶消化、假阳性及假阴性的产生等进行了概述,旨在通过探讨上述问题,对该技术的实际应用有所指导。

2) In-well in situ RT-PCR 点击朗读

液相原位反转录PCR

3) RT-PCR 点击朗读

反转录PCR

Methods: The expression of 5-HT receptor subtype mRNAs was detected in the lumbar dorsal root ganglion (DRG) by reverse transcription-polymerase chain reaction(RT-PCR) following unilateral injection of formalin into the plantar surface of rat hind paw.

方法:用反转录PCR技术观察大鼠单侧足底皮下注射福尔马林致痛后背根节内5-HT1~7受体亚型mRNAs的表达变化。

?Methods Using the total RNA extracted from fresh rat hippocampus as template,the cDNA of PDZ1 was amplified by RT-PCR;the cDNA obtained was subsequently cloned into the shuttle vector pAdTrack-CMV(CLONTECH),and then,the sequence of the product was determined.

方法以异硫酸氢胍-酚-氯仿一步法提取的总RNA为模板,采用反转录PCR(RT-PCR)法获得PDZ1的cDNA;与腺病毒穿梭载体用T4DNA连接酶连接;连接产物转化大肠杆菌JM109进行筛选、序列测定。

Methods:BMP7 gene was cloned from the HEK293 cells with RT-PCR and nested-PCR skills,and after being repaired,the whole cDNA of BMP7 was obtained.

方法 :通过反转录PCR技术和巢式PCR技术从人类胚胎肾 2 93细胞中克隆BMP7基因 ,应用互补拼接的方法对其进行修复获得全长cDNA ,将其与T载体进行连接得到BMP7克隆质粒。

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4) RT-PCR 点击朗读

反转录-PCR

0kb DNA fragment was obtained by RT-PCR fr. 点击朗读

本研究以黑曲霉M-1为出发菌株，设计特异的引物，采用反转录-PCR技术直接从黑曲霉总RNA中反转录并扩增出约3。

5) RT PCR 点击朗读

反转录PCR

cDNA fragment encoding human P34H was amplified by RT PCR using specific primers, and then was cloned into pGEM T vector.

　方法 :提取人附睾体部总RNA ,并以此为模板 ,进行反转录PCR获得编码P34H蛋白的基因片段。

Methods:Tumor tissues and adjacent normal tissues in 41 cases of esophageal carcinoma were studied by using the reverse transcriptase polymerase chain reaction(RT PCR).

方法 :用反转录PCR(RT PCR)法检测 41例食管癌患者的癌组织、癌旁组织MMP 2mRNA的表达。

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6) Reverse transcription PCR 点击朗读

反转录PCR

例句>>

补充资料：反转录PCR

分子式：
CAS号：

性质：以RNA为起始对象，经反转录酶作用合成cDNA后，再用DNA引物进行DNA扩增，两个反向连续进行，叫作反转录PCR。

说明：补充资料仅用于学习参考，请勿用于其它任何用途。

参考词条

反转录-二次PCR 反转录PCR分析半定量反转录PCR 实时反转录PCR 逆转录PCR 逆转录-PCR 实时定量反转录PCR

原位逆转录PCR技术反转录巢式PCR 反转录套式PCR

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	您的位置：首页 -> 词典 -> 原位反转录PCR 1) in situ RT-PCR 原位反转录PCR 1. The principle, key factors and steps of in situ RT-PCR including tissues or cells fixation, primer and probe selections, protease or DNase digestions, false positive and false negative results were summarized to emphasize the application of in situ RT-PCR to the actual detections. 针对已有资料中对于以DNA为靶基因序列的原位 PCR介绍较多,而对于原位反转录PCR的介绍很少,但在实际研究工作中有很多检测的靶基因是 RNA(病毒的或基因组的 RNA)的实际情况,就原位反转录PCR技术的基本原理、影响试验结果的关键因素和步骤,如组织细胞的固定、引物和探针的选择、蛋白酶及DNA酶消化、假阳性及假阴性的产生等进行了概述,旨在通过探讨上述问题,对该技术的实际应用有所指导。 2) In-well in situ RT-PCR 液相原位反转录PCR 3) RT-PCR 反转录PCR 1. Methods: The expression of 5-HT receptor subtype mRNAs was detected in the lumbar dorsal root ganglion (DRG) by reverse transcription-polymerase chain reaction(RT-PCR) following unilateral injection of formalin into the plantar surface of rat hind paw. 方法:用反转录PCR技术观察大鼠单侧足底皮下注射福尔马林致痛后背根节内5-HT1~7受体亚型mRNAs的表达变化。 2. ?Methods Using the total RNA extracted from fresh rat hippocampus as template,the cDNA of PDZ1 was amplified by RT-PCR;the cDNA obtained was subsequently cloned into the shuttle vector pAdTrack-CMV(CLONTECH),and then,the sequence of the product was determined. 方法以异硫酸氢胍-酚-氯仿一步法提取的总RNA为模板,采用反转录PCR(RT-PCR)法获得PDZ1的cDNA;与腺病毒穿梭载体用T4DNA连接酶连接;连接产物转化大肠杆菌JM109进行筛选、序列测定。 3. Methods:BMP7 gene was cloned from the HEK293 cells with RT-PCR and nested-PCR skills,and after being repaired,the whole cDNA of BMP7 was obtained. 方法 :通过反转录PCR技术和巢式PCR技术从人类胚胎肾 2 93细胞中克隆BMP7基因 ,应用互补拼接的方法对其进行修复获得全长cDNA ,将其与T载体进行连接得到BMP7克隆质粒。更多例句>> 4) RT-PCR 反转录-PCR 1. 0kb DNA fragment was obtained by RT-PCR fr. 本研究以黑曲霉M-1为出发菌株，设计特异的引物，采用反转录-PCR技术直接从黑曲霉总RNA中反转录并扩增出约3。 5) RT PCR 反转录PCR 1. cDNA fragment encoding human P34H was amplified by RT PCR using specific primers, and then was cloned into pGEM T vector. 　方法 :提取人附睾体部总RNA ,并以此为模板 ,进行反转录PCR获得编码P34H蛋白的基因片段。 2. Methods:Tumor tissues and adjacent normal tissues in 41 cases of esophageal carcinoma were studied by using the reverse transcriptase polymerase chain reaction(RT PCR). 方法 :用反转录PCR(RT PCR)法检测 41例食管癌患者的癌组织、癌旁组织MMP 2mRNA的表达。更多例句>> 6) Reverse transcription PCR 反转录PCR 例句>> 补充资料：反转录PCR 分子式： CAS号：性质：以RNA为起始对象，经反转录酶作用合成cDNA后，再用DNA引物进行DNA扩增，两个反向连续进行，叫作反转录PCR。说明：补充资料仅用于学习参考，请勿用于其它任何用途。参考词条反转录-二次PCR 反转录PCR分析半定量反转录PCR 实时反转录PCR 逆转录PCR 逆转录-PCR 实时定量反转录PCR

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