1) real-time reverse transcription PCR
实时反转录PCR
2) Real Time RT-PCR
实时定量反转录PCR
1.
[Method] Tissue specific expression of HIF-1α gene in Qinghai domestic yak was detected using the methods of Semi-quantitative RT-PCR and Real Time RT-PCR.
[方法]应用半定量反转录PCR和实时定量反转录PCR(SYBRGreen)技术对青海家牦牛HIF-1α基因的组织特异性表达进行检测。
3) real-time fluorescent quantitative reverse transcription polymerase chain reaction
实时荧光定量反转录PCR
4) real-time reverse transcription polymerase chain reaction(RT-RTPCR)
反转录实时荧光定量PCR
5) real-time reverse transcription-PCR
实时荧光定量反转录-PCR
1.
mRNA accumulation of tcbC in J5-2 was quantified by real-time reverse transcription-PCR,when J5-2 grew on 1,2-dichlorobenzene,1,3-dichlorobenzene and 1,2,4-trichlorobenzene respectively.
利用实时荧光定量反转录-PCR(real-time reverse transcription-PCR)方法,考察了菌株J5-2在分别以1,2-二氯苯、1,3-二氯苯和1,2,4-三氯苯为唯一碳源时tcbC(J5-2)基因的定量表达。
6) RT-PCR
反转录PCR
1.
Methods: The expression of 5-HT receptor subtype mRNAs was detected in the lumbar dorsal root ganglion (DRG) by reverse transcription-polymerase chain reaction(RT-PCR) following unilateral injection of formalin into the plantar surface of rat hind paw.
方法:用反转录PCR技术观察大鼠单侧足底皮下注射福尔马林致痛后背根节内5-HT1~7受体亚型mRNAs的表达变化。
2.
?Methods Using the total RNA extracted from fresh rat hippocampus as template,the cDNA of PDZ1 was amplified by RT-PCR;the cDNA obtained was subsequently cloned into the shuttle vector pAdTrack-CMV(CLONTECH),and then,the sequence of the product was determined.
方法以异硫酸氢胍-酚-氯仿一步法提取的总RNA为模板,采用反转录PCR(RT-PCR)法获得PDZ1的cDNA;与腺病毒穿梭载体用T4DNA连接酶连接;连接产物转化大肠杆菌JM109进行筛选、序列测定。
3.
Methods:BMP7 gene was cloned from the HEK293 cells with RT-PCR and nested-PCR skills,and after being repaired,the whole cDNA of BMP7 was obtained.
方法 :通过反转录PCR技术和巢式PCR技术从人类胚胎肾 2 93细胞中克隆BMP7基因 ,应用互补拼接的方法对其进行修复获得全长cDNA ,将其与T载体进行连接得到BMP7克隆质粒。
补充资料：反转录PCR
分子式：
CAS号：
性质：以RNA为起始对象，经反转录酶作用合成cDNA后，再用DNA引物进行DNA扩增，两个反向连续进行，叫作反转录PCR。
CAS号：
性质：以RNA为起始对象，经反转录酶作用合成cDNA后，再用DNA引物进行DNA扩增，两个反向连续进行，叫作反转录PCR。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条