1) Inverse nestd PCR
反向嵌套巢式PCR
2) inverse nested PCR
反向嵌套PCR
1.
In order to study the role and functions in the process of growth and development of the reindeer ghrelin,inverse nested PCR was used for amplifying 5′cDNA end with a specific RT primer which 5′end is phosphated and two pairs of specific inverse nested PCR primers designed basing on the known cDNA sequence of reindeer ghrelin.
为了研究Ghrelin在驯鹿生长发育过程中的作用和功能,以驯鹿为研究对象,采用反向嵌套PCR RACE技术原理,根据已知的序列设计1条5′末端磷酸化的特异性反转录引物和2对特异性反向嵌套PCR引物,首先进行反转录(RT),然后将反转录成的cDNA进行环化,最后进行反向嵌套巢式PCR,成功地扩增了驯鹿Ghrelin cDNA 5′末端序列。
2.
In addition,Inverse nested PCR can rapidly amplify 5′ cDNA end by using a specific RT primer whose 5′ end is phosphated and two pairs specific inverse nested.
另外,采用反向嵌套PCRRACE法,根据caBD_1cDNA的已知序列,设计一条5′末端磷酸化的特异性反转录引物和两对特异性反向嵌套PCR引物,首先进行反转录(RT),然后将mRNA反转录成的cDNA进行环化,最后进行反向嵌套巢式PCR,成功的克隆了骆驼β_防御素caBD_1cD NA的5′末端序列。
3.
Inverse nested PCR can rapidly amplify 5′cDNA ends by using a specific RT primer whose 5′end is phosphated and two pairs specific Inverse nested PCR primers.
采用反向嵌套PCR法 ,根据已获得的辽宁碱蓬甜菜碱醛脱氢酶cDNA的部分序列 ,设计一条 5′末端磷酸化的特异性反转录引物和两对特异性反向嵌套PCR引物 ,成功地克隆了辽宁碱蓬甜菜碱醛脱氢酶cDNA 5′末端。
3) nested inverse PCR
巢式反向PCR
4) nested PCR
嵌套式PCR
1.
Nested PCR for Ultrasensitive Detection of the Potato Ring Rot Bacterium;
嵌套式PCR超灵敏检测马铃薯环腐病菌
5) heminested PCR
半嵌套式PCR
1.
The heminested PCR results showed that the foreign genes of the seven positi.
半嵌套式PCR检测结果表明阳性样品中的外源目的基因为来自根癌农杆菌CP4菌株的EPSPS基因。
6) RT-nested PCR
反转录巢式PCR
补充资料:嵌套PCR
分子式:
CAS号:
性质:后一次PCR的引物互补于前一次PCR所扩增序列的内侧。
CAS号:
性质:后一次PCR的引物互补于前一次PCR所扩增序列的内侧。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条