1) RT-PCR analysis
反转录PCR分析
2) RT-PCR
反转录PCR
1.
Methods: The expression of 5-HT receptor subtype mRNAs was detected in the lumbar dorsal root ganglion (DRG) by reverse transcription-polymerase chain reaction(RT-PCR) following unilateral injection of formalin into the plantar surface of rat hind paw.
方法:用反转录PCR技术观察大鼠单侧足底皮下注射福尔马林致痛后背根节内5-HT1~7受体亚型mRNAs的表达变化。
2.
?Methods Using the total RNA extracted from fresh rat hippocampus as template,the cDNA of PDZ1 was amplified by RT-PCR;the cDNA obtained was subsequently cloned into the shuttle vector pAdTrack-CMV(CLONTECH),and then,the sequence of the product was determined.
方法以异硫酸氢胍-酚-氯仿一步法提取的总RNA为模板,采用反转录PCR(RT-PCR)法获得PDZ1的cDNA;与腺病毒穿梭载体用T4DNA连接酶连接;连接产物转化大肠杆菌JM109进行筛选、序列测定。
3.
Methods:BMP7 gene was cloned from the HEK293 cells with RT-PCR and nested-PCR skills,and after being repaired,the whole cDNA of BMP7 was obtained.
方法 :通过反转录PCR技术和巢式PCR技术从人类胚胎肾 2 93细胞中克隆BMP7基因 ,应用互补拼接的方法对其进行修复获得全长cDNA ,将其与T载体进行连接得到BMP7克隆质粒。
3) RT-PCR
反转录-PCR
1.
0kb DNA fragment was obtained by RT-PCR fr.
本研究以黑曲霉M-1为出发菌株，设计特异的引物，采用反转录-PCR技术直接从黑曲霉总RNA中反转录并扩增出约3。
4) RT PCR
反转录PCR
1.
cDNA fragment encoding human P34H was amplified by RT PCR using specific primers, and then was cloned into pGEM T vector.
方法 :提取人附睾体部总RNA ,并以此为模板 ,进行反转录PCR获得编码P34H蛋白的基因片段。
2.
Methods:Tumor tissues and adjacent normal tissues in 41 cases of esophageal carcinoma were studied by using the reverse transcriptase polymerase chain reaction(RT PCR).
方法 :用反转录PCR(RT PCR)法检测 41例食管癌患者的癌组织、癌旁组织MMP 2mRNA的表达。
补充资料：反转
反转——
股价朝原来趋势的相反方向移动分为向上反转和向下反转。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条