1) improved CTAB method
改良的CTAB法
1.
To find a normal method for extracting genomic DNA from planaria, the improved CTAB method, SDS method, SDS-Proteinase K method were used to extract genomic DNA from Dugesia japonica.
为了找到一种适用于提取涡虫基因组DNA的常规方法,我们以东亚三角头涡虫为材料,分别用改良的CTAB法、SDS法、SDS-蛋白酶K法对涡虫的基因组DNA进行了制备,并对3种方法制备的涡虫基因组DNA进行了检测与比较。
2.
The experiment results showed that the improved CTAB method was suitable for all kinds of molecular analysis due to its simplicity and high efficiency.
采用改良的CTAB法,植物基因组DNA小量制备试剂盒,SDS法和高盐低pH值法4种方法提取安徽市场上8种大豆种子的基因组DNA,首次用降落PCR(TD-PCR)检测转基因成分,并对阳性产物进行酶切验证。
2) modified CTAB method
改良CTAB法
1.
Methods: Two different methods,conventional CTAB method and modified CTAB method,were applied to isolate genomic DNA from dry leaves of Lonicera Japonica Thunb.
方法:分别采用常规CTAB法与改良CTAB法提取干叶的DNA,通过琼脂糖凝胶电泳、紫外分光光度法、酶切检测其质量,并与鲜叶的提取效果比较。
2.
To satisfy their increasing researches in molecular Biology, modified CTAB and different concentration gradient of EDTA with 2-mercaptoethanol in modified CTAB method are described in the present paper.
采用改良CTAB法以及不同 2 -巯基乙醇梯度和不同EDTA梯度分离基因组DNA的方法对多糖含量高、极易褐化的美味猕猴桃嫩叶基因组DNA提取条件进行了优化。
3.
Method:Two different methods,conventional CTAB method and modified CTAB method,were applied to extract genomic DNA from leaves of Lonicera japonica Thunb.
方法:采用改良CTAB法从忍冬叶片中提取DNA,通过琼脂糖凝胶电泳、紫外分光光度法、酶切检测其质量,并与常规CTAB法比较。
3) Improved CTAB Method
改良CTAB法
1.
The result showed that DNAs extracted by the improved CTAB method are much purer than those of SDS method.
采用改良CTAB法和SDS法提取椰子基因组DNA,并通过琼脂糖凝胶电泳、分光光度计进行紫外检测。
2.
Method: The improved CTAB method and improved SDS method were used to extract DNA.
方法:采用改良CTAB法和改良SDS法提取澳洲坚果样品的总DNA,并对产物进行紫外、电泳及PCR扩增检测。
3.
By studying the appearance,concentration,A260/A280,A260/A230 and electrophoresis,and comparing the total DNA extracted from ISSR analysis,the result showed that the improved CTAB method was suitable for ISSR analysis,and it was a better DNA extraction method of Casta nopsis fissa.
对大叶栎的总DNA两种提取方法进行对比试验,从外观、浓度、A260/A280、A260/A230、电泳检查和ISSR扩增对所提取到的DNA进行对比,结果显示用改良CTAB法提取的DNA在各方面都达到ISSR扩增的要求,是大叶栎总DNA提取的较好方法。
4) modified CTAB-LiCl
改良CTAB-LiCl法
1.
The results showed that the modified CTAB-LiCl was capable of efficiently removing polysaccharides,and that of the RNAs extracted by the modified CTAB-LiCl,the lightness of 28 s rRNA was two times as much as that of 18 S Rrna, the value of A260/A280,A260/A230 was1.
结果表明,改良CTAB-LiCl法能有效地去除多糖,提取到的RNA 28 SrRNA亮度约为18SrRNA的二倍,A260/A280比值为1。
5) modified CTAB method
改进的CTAB法
1.
The results showed that the modified CTAB method was better than regular method in terms of the quality of total DNA extraction.
用改进的CTAB法提取猪毛菜、碱蓬、骆驼蓬、骆驼蹄瓣和柽柳5种盐生植物基因组DNA,通过与常规的CTAB法提取结果相比发现,改进的CTAB法从提取速度、得率、质量上都优于常规的CTAB法,并总结出一套适用于盐生植物的快速高质量基因组DNA的提取方法。
6) modified CTAB
改良CTAB
1.
This experiment made use of the modified CTAB,carried on Mararate isolation and purification of DNA from various corn samples.
利用改良CTAB法提取玉米产品的基因组DNA,应用PCR检测方法,并以玉米特异性内源基因IVR为内参,花椰菜花叶病毒35S启动子(CaMV35S启动子)、农杆菌胭脂碱合成酶终止子(NOS终止子)为靶基因检测玉米中是否存在转基因成分。
2.
Experiment was carried out on isolation of total RNA from flower-bud of Carya cathayensis by the methods of modified CTAB,guanidine isothiocyanate and modified hot phenol.
以山核桃的花芽为材料,利用改良CTAB法、异硫氰酸胍法和改良热酚法提取其总RNA,并对RNA产率、纯度、电泳图谱和RT-PCR进行分析,结果表明:改良CTAB法明显优于其它两种提取方法,用它提取的28S rRNA、18S rRNA条带清晰、稳定,无降解,OD260/OD280值保持在1。
补充资料：改良型曝气法
分子式：
CAS号：
性质：废水活性污泥法的一种变型。流程与常规活性污泥法相同，但采用缩短曝气时间(1.5～3h)，并减少混合液中悬浮固体的含量(200≈500mg/L MLSS)。其污泥沉降性一般较差，出水中悬浮固体的含量较高。此法可用作中等程度的处理，只需设置格栅和沉砂池作为预处理，不需设初次沉淀池。其污泥回流率小(5％～15％)，可减少回流污泥动力消耗。
CAS号：
性质：废水活性污泥法的一种变型。流程与常规活性污泥法相同，但采用缩短曝气时间(1.5～3h)，并减少混合液中悬浮固体的含量(200≈500mg/L MLSS)。其污泥沉降性一般较差，出水中悬浮固体的含量较高。此法可用作中等程度的处理，只需设置格栅和沉砂池作为预处理，不需设初次沉淀池。其污泥回流率小(5％～15％)，可减少回流污泥动力消耗。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条