1) CTAB-LiCl method
CTAB-LiCl法
1.
The results show the higher yield and good quality of RNA are extracted using an improved CTAB-LiCl method which can be used directly in cDNA synthesis, cDNA-AFLP analysis and other molecular biological experiment.
试验以茶树种胚为研究对象,通过对几种提取总RNA方法的比较与分析,认为用改进的CTAB-LiCl法提取茶树种胚总RNA的产率较高,质量好,可直接用于cDNA的合成、cDNA-AFLP分析等分子生物学实验操作。
2.
Total RNA extracted by CTAB-LiCl method had high quality, which is the best method of total RNA isolation from Dioscorea opposita.
采用异硫氰酸胍-巯基乙醇联合变性法、Trizol法和CTAB-LiCl法等3种方法,分别对山药叶片和块茎进行总RNA提取效果进行了比较。
2) CTAB-LiCl-based extraction method
CTAB-LiCl提取法
1.
A novel and simple CTAB-LiCl-based extraction method for high-quality and total RNA of rice embryo samples is developed.
介绍了一种全新、简单的提取水稻种胚RNA的CTAB-LiCl提取法,可在不用液氮的室温下有效地排除剥取的种胚中大量带有的多糖和本身脂质的干扰,所得RNA样品经紫外分光光度计和琼脂糖凝胶电泳分析证明具有较高的纯度和完整性,并可进一步满足RT-PCR和Northern印迹的实验需要,适用于富含多糖和脂质的植物组织RNA的提取。
3) modified CTAB-LiCl
改良CTAB-LiCl法
1.
The results showed that the modified CTAB-LiCl was capable of efficiently removing polysaccharides,and that of the RNAs extracted by the modified CTAB-LiCl,the lightness of 28 s rRNA was two times as much as that of 18 S Rrna, the value of A260/A280,A260/A230 was1.
结果表明,改良CTAB-LiCl法能有效地去除多糖,提取到的RNA 28 SrRNA亮度约为18SrRNA的二倍,A260/A280比值为1。
4) LiCl method
LiCl法
1.
To look for rapid and efficient methods to isolate DNA,three algae(Porphyra yezoensis,Enteromorpha clathrata and Spirulina platensis)were as materials to extract DNA by three preparation methods,improved CTAB method,LiCl method and extraction kit.
选取三种不同海藻纲中的条斑紫菜、条浒苔、钝顶螺旋藻为材料,通过采用改良的CTAB法、LiCl法及试剂盒法抽提基因组DNA并进行比较,以找出适用于各种藻类DNA提取的快速有效方法。
5) CTAB
CTAB法
1.
Study on Efficient Extraction for Genomic DNA from Malus Leaves by CTAB Method
CTAB法高效提取苹果叶片DNA的研究
2.
Method The samples were extracted by the classic technique of CTAB and improved CTAB.
方法采用经典CTAB法与改良CTAB法。
3.
We adopted the phenol-chloroform-isopentanol, CTAB and SDS methods to extract DNA from Sinononacula constricta , Meretix linnaeus,Chlamys nobilis, Haliotis diversicolor and other shellfishes.
以缢蛏、美人蛏、文蛤、扇贝和鲍鱼等几种经济贝类为研究对象,分别用苯酚/氯仿/异戊醇法,CTAB法,SDS法等不同方法提取其基因组DNA,寻找适宜于研究对象基因组DNA提取的方法,结果表明,3种方法均可以从目标材料中提取到DNA,但苯酚/氯仿/异戊醇法无论从数量和质量上都不理想,而SDS和CTAB可根据具体情况选择使用。
6) CTAB method
CTAB法
1.
DNA extraction of Staphylococcus aureus using the CTAB method;
CTAB法在金黄色葡萄球菌DNA提取中的应用
2.
Isolation of genomic DNA from different organs in Anthurium andraeanum by CTAB method;
利用CTAB法提取红掌不同部位基因组DNA
3.
Research of optimizing CTAB method in DNA extraction of cycad using orthogonal experiment design;
CTAB法提取苏铁基因组DNA的条件优化
补充资料:licl
CAS:7447-41-8
分子式:ClLi
分子质量:42.39
中文名称:氯化锂;氯化锂(无水);氯化锂(结晶);氯化锂,无水
英文名称:Lithium chloride;Lithium chloride, anhydrous;chlorku litu;chlorure de lithium;licl;lithium chloride (licl);Lithiumchlorid
分子式:ClLi
分子质量:42.39
中文名称:氯化锂;氯化锂(无水);氯化锂(结晶);氯化锂,无水
英文名称:Lithium chloride;Lithium chloride, anhydrous;chlorku litu;chlorure de lithium;licl;lithium chloride (licl);Lithiumchlorid
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条