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1)  RT-PCR
反转录-PCR
1.
0kb DNA fragment was obtained by RT-PCR fr.
本研究以黑曲霉M-1为出发菌株，设计特异的引物，采用反转录-PCR技术直接从黑曲霉总RNA中反转录并扩增出约3。
2)  RT-PCR
反转录PCR
1.
Methods: The expression of 5-HT receptor subtype mRNAs was detected in the lumbar dorsal root ganglion (DRG) by reverse transcription-polymerase chain reaction(RT-PCR) following unilateral injection of formalin into the plantar surface of rat hind paw.
方法:用反转录PCR技术观察大鼠单侧足底皮下注射福尔马林致痛后背根节内5-HT1~7受体亚型mRNAs的表达变化。
2.
?Methods Using the total RNA extracted from fresh rat hippocampus as template,the cDNA of PDZ1 was amplified by RT-PCR;the cDNA obtained was subsequently cloned into the shuttle vector pAdTrack-CMV(CLONTECH),and then,the sequence of the product was determined.
方法以异硫酸氢胍-酚-氯仿一步法提取的总RNA为模板,采用反转录PCR(RT-PCR)法获得PDZ1的cDNA;与腺病毒穿梭载体用T4DNA连接酶连接;连接产物转化大肠杆菌JM109进行筛选、序列测定。
3.
Methods:BMP7 gene was cloned from the HEK293 cells with RT-PCR and nested-PCR skills,and after being repaired,the whole cDNA of BMP7 was obtained.
方法 :通过反转录PCR技术和巢式PCR技术从人类胚胎肾 2 93细胞中克隆BMP7基因 ,应用互补拼接的方法对其进行修复获得全长cDNA ,将其与T载体进行连接得到BMP7克隆质粒。
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3)  RT PCR
反转录PCR
1.
cDNA fragment encoding human P34H was amplified by RT PCR using specific primers, and then was cloned into pGEM T vector.
　方法 :提取人附睾体部总RNA ,并以此为模板 ,进行反转录PCR获得编码P34H蛋白的基因片段。
2.
Methods:Tumor tissues and adjacent normal tissues in 41 cases of esophageal carcinoma were studied by using the reverse transcriptase polymerase chain reaction(RT PCR).
方法 :用反转录PCR(RT PCR)法检测 41例食管癌患者的癌组织、癌旁组织MMP 2mRNA的表达。
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4)  Reverse transcription PCR
反转录PCR
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5)  reverse-transcription PCR
反转录PCR
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6)  in situ RT-PCR
原位反转录PCR
1.
The principle, key factors and steps of in situ RT-PCR including tissues or cells fixation, primer and probe selections, protease or DNase digestions, false positive and false negative results were summarized to emphasize the application of in situ RT-PCR to the actual detections.
针对已有资料中对于以DNA为靶基因序列的原位 PCR介绍较多,而对于原位反转录PCR的介绍很少,但在实际研究工作中有很多检测的靶基因是 RNA(病毒的或基因组的 RNA)的实际情况,就原位反转录PCR技术的基本原理、影响试验结果的关键因素和步骤,如组织细胞的固定、引物和探针的选择、蛋白酶及DNA酶消化、假阳性及假阴性的产生等进行了概述,旨在通过探讨上述问题,对该技术的实际应用有所指导。
补充资料：反转录PCR
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性质：以RNA为起始对象，经反转录酶作用合成cDNA后，再用DNA引物进行DNA扩增，两个反向连续进行，叫作反转录PCR。

说明：补充资料仅用于学习参考，请勿用于其它任何用途。
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