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1)  hair-pin RNA
发夹RNA ( hpRNA)
2)  hairpin RNA (hpRNA)
发卡RNA(hpRNA)
3)  Short hairpin RNA
短发夹RNA
1.
Construction and identification of a vector expressing short hairpin RNA targeting annexinⅡgene;
膜联蛋白Ⅱ基因短发夹RNA表达载体的构建和鉴定
2.
The effect of short hairpin RNA on hTERT expression;
短发夹RNA对人端粒酶催化亚基表达的影响
3.
Objective To clone shRNA (short hairpin RNA) recombinant plasmid targeting on COX-2 gene and analyze the nucleic acid sequence of the recombinant plasmid.
目的利用RNA干扰技术,以COX-2为靶基因,构建靶向COX-2基因的短发夹RNA(shRNA)重组表达质粒并进行鉴定分析。
4)  shRNA
短发夹RNA
1.
Inhibition of ICAM-1 gene expression in liver cancer cells by shRNA;
短发夹RNA对肝癌细胞细胞间粘附分子-1基因表达的影响
2.
Objectives To investigate nano-carrier G9 PAMAM mediated plasmid containing CTGF-shRNA (pCTGF-shRNA) transfection on connective tissue growth factor (CTGF) expression in mouse peritoneal mesothelial cells (MPMCs).
目的研究G9PAMAM纳米载体介导的结缔组织生长因子(CTGF)短发夹RNA(shRNA)质粒(pCTGF-shRNA)对小鼠腹膜间皮细胞CTGF表达的影响。
3.
The annealed oligonucleotides were inserted into the linearized pSIREN-RetroQ vector to construct the recombinant shRNA plasmid(pSIREN-VEGF-C) and then transformed into E.
方法:根据GenBank中VEGF-C序列,设计、合成靶向VEGF-C基因、编码短发夹RNA的两条寡核苷酸序列,退火后用T4连接酶与线性化的pSIREN载体连接,转化感受态大肠杆菌,筛选阳性克隆,抽提质粒,获得重组pSIREN-VEGF-C质粒;BglⅡ和EcoRⅠ双酶切、测序鉴定。
5)  small hairpin RNA
小发夹RNA
1.
Now there are four main kinds of RNA interference libraries with different construction approaches and molecular form,chemically synthesized small interference RNA(siRNA) libraries,chemically synthesized small hairpin RNA(shRNA) libraries,enzymatically prepared siRNA libraries and enzymatically prepared shRNA libraries.
不同种类的RNA干扰库已经被用于基因功能的研究之中,根据其构建方式和分子形式的不同,可将RNA干扰库分为4种类型,即化学合成的小干扰RNA(siRNA)库、化学合成的小发夹RNA(shRNA)表达库、酶切法构建的siRNA库和酶切法构建的shRNA表达库。
2.
[Methods] A shRNA expressed vector that expresses the specific small hairpin RNA targeting TRAP mRNA was constructed and transfected into SGC-7901 cells, and the stably expressing shRNA cells were selected by G418 and continuously cultured in half the antibiotic concentration for 3 months.
目的探讨稳定转染靶向端粒酶调节相关基因TRAP的小发夹RNA对胃癌细胞株SGC-7901的长效影响。
3.
[Methods] Plasmid vectors expressing small hairpin RNA (shRNA) targeting at cyclin D1 gene were constructed and transfected into K562 cells mediated by chitosan.
[方法]体外构建靶向cyclin D1基因的小发夹RNA(shRNA)表达质粒,通过壳聚糖介导转染K562细胞,Western blot分析检测转染前后cyclin D1蛋白表达变化;集落形成实验检测细胞增殖能力;流式细胞仪检测细胞周期分布及凋亡情况。
6)  short hairpin RNA(shRNA)
短发夹RNA
1.
Objective: To transfect a recombinant short hairpin RNA(shRNA)expression vector targeting Livin gene isoform(BIRC71,BIRC72)into the cervical cancer cell line(Hela cell),in an attempt to observe RNAi-mediated silen- cing on Livin gene and the induction of Hela apoptosis.
目的:应用构建成功的Livin异构体(BIRC71,BIRC72)短发夹RNA(short hairpin HNA,shRNA)真核表达载体转染Hela细胞,探讨其对Livin基因的沉默效应及诱导Hela细胞凋亡的作用。
补充资料:感染性RNA病原RNA
分子式:
CAS号:

性质:又称感染性RNA病原RNA;壳病毒,是一种和病毒(virus)相似的感染性颗粒。为无蛋白外壳的单链RNA,分子量1.1×105~1.3×105。它是比已知病毒都小的能在宿主细胞内自主复制的病原体之一。已知的近20种类病毒中,大部分已测得了一级结构,都是无蛋白外壳的共价闭合的单链环状RNA分子。在天然状态下类病毒RNA以高度碱基配对的棒状结构形式存在。最先是由T. O. Diener等人(1969)在马铃薯纤块茎病(potato spindle tuber disease)的病株上首先发现的,在电镜下可见到这RNA分子呈50nm长的杆状分子,共有359个碱基对,并证实是游离的RNA,为此正式命名为类病毒。它通常在宿主细胞核内,借助汁液传染,分子量75000~130000,比最小病毒还小80倍。后又相继在菊花矮缩病(chrysanthemum stunt)、菊花绿斑病(chrysanthenum chlorotic mottle)、柑橘剥皮病(citrus excortis)等患病植株中分离到低分子量的病原RNA。推测它也可能存在于其他植物、动物甚至人体内。绝大部分类病毒均具有共同的结构特征:(1)位于棒状结构中心有一个高度保守的序列;(2)靠近这一保守中心区的左侧有一个多聚嘌呤区;(3)棒状结构左侧序列保守性强,右侧变异性大。它可能是通过核苷酸序列或结构改变直接与寄主细胞相互作用、干扰细胞的代谢而致病。对类病毒的研究可能为揭示生命起源和进化、生命过程的实现等生命科学的重大理论问题作出贡献。

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