1) nested PCR
巢式PCR
1.
Amplify of fetal SRY gene in maternal plasma by using nested PCR;
应用巢式PCR扩增孕妇血浆中胎儿SRY基因
2.
Cloning of N-terminal deleted IκBα gene by nested PCR;
应用巢式PCR法克隆N端部分缺失的IκBα基因
3.
Establishment of a nested PCR assay for detection of Mycoplasma ovis;
羊附红细胞体巢式PCR检测方法的建立及临床应用
2) nested-PCR
巢式PCR
1.
Nested-PCR detection of human cytomegalovirus, Epstein-Barr virus, herpes simplex virus type 1 in the subgingival plaques and association with chronic periodontitis;
巢式PCR检测龈下菌斑中HCMV、EBV、HSV-1与慢性牙周炎活动性的关系
2.
Detection of Fusarium oxysporum f.sp.cucumarinum by PCR-RFLP and Nested-PCR;
应用PCR-RFLP和巢式PCR检测黄瓜尖镰孢菌
3.
Detection of Fusarium oxysporum f.sp.cucumbrum by nested-PCR;
应用巢式PCR检测黄瓜尖镰孢菌(Fusarium oxysporum f．sp．cucumbrum)
3) Nest-PCR
巢式PCR
1.
In this study, copepoda and its resting eggs in shrimp culture ponds were detected using PCR dot-blot hybridization and nest-PCR for the presence of WSSV.
本研究利用PCR-斑点杂交和巢式PCR法检测野外发病对虾养殖池塘中桡足类及其休眠卵,以及由这些休眠卵孵化出的幼体携带病毒情况,发现桡足类由卵到成体各阶段中均有阳性个体,而且池塘中在春天放养对虾苗前桡足类就出现阳性个体。
2.
Detection of Pneumonia Chlamydiae in the Peripheral Blood Mononuclear Cell of Coronary Heart Disease by Indirect Immunofluorescence and Nest-PCR;
方法:选择150例冠心病(冠心病组)及55例非冠心病(对照组)患者,使用低渗溶血法制备PBMC,直接免疫荧光(DIF)检测Cpn包涵体,巢式PCR(nPCR)法测定外周血单核细胞中肺炎衣原体DNA。
4) Nest PCR
巢式PCR
1.
A new method for identifing the change of single nucleotide by created restriction site PCR and Nest PCR;
联合应用巢式PCR和创造酶切位点法检测单核苷酸改变
2.
Utilization of Nest PCR in Intestinal Infection Caused by F subgenus of Enteric Adenovirus
巢式PCR在腺病毒F亚属引起的致腹泻肠道感染检测中的应用
3.
Methods The expressive levels of α1 integrin in specimens were determined by FQ-PCR technique and nest PCR.
方法应用荧光定量PCR和巢式PCR技术检测10例增生性瘢痕组织中整合素α1的表达,并以10例正常皮肤组织作为对照。
5) nested polymerase chain reaction
巢式PCR
1.
Sexing of early sheep embryos by using nested polymerase chain reaction;
绵羊早期胚胎性别鉴定巢式PCR体系的建立及其灵敏度研究
2.
Methods Tissue blocks of lymph nodes from 32 true HNL patients and 13 normal subjects were confirmed for parvovirus B19 infection by nested polymerase chain reaction and immunohistochemical techniques in a double blind manner.
方法采用双盲法在32例真性HNL患者及13例正常对照淋巴结活检组织中用巢式PCR和免疫组化染色检测B19病毒基因组DNA和病毒蛋白。
3.
Methods The specific HHV-7 sequences were examined with a nested polymerase chain reaction(PCR) procedure in the lesional skin, plasma, peripheral blood mononuclear cells (PBMC), saliva, and urine samples of 22 cases of acute pityriasis rosea patients as well as in the saliva, plasma and PBMC of 14 convalescent pityriasis rosea patients.
方法采用巢式PCR检测了22例PR急性期患者的血浆、外周血单核细胞(PBMC)、皮损、唾液、尿液,14例恢复期患者的唾液、血浆、外周血单核细胞,14例正常人的唾液、血浆、外周血单核细胞中的HHV-7特异性序列。
6) Nested RT-PCR
巢式RT-PCR
1.
Development of nested RT-PCR for detection animal rabies virus
动物狂犬病病毒巢式RT-PCR检测方法的建立
2.
To study the incidence, the types of fusion genes and the clinical significance of rearrangements of mixed lineage leukemia (MLL) gene in acute leukemia (AL), the rearrangements of MLL gene of 60 patients with AL were detected by fluorescence in situ hybridization (FISH) and 6 types of common fusion genes resulting from the rearrangements of MLL gene were detected by nested RT-PCR.
为了研究混合系白血病(MLL)基因重排在急性白血病(AL)中的发生率、融合基因类型及其临床意义,用荧光原位杂交技术检测60例急性白血病(AL)患者MLL基因重排,对于MLL基因重排阳性的患者,用巢式RT-PCR方法检测MLL基因重排形成的6种常见融合基因类型。
3.
Methods CK19mRNA expression was detected by nested RT-PCR in the peripheral blood from 60 lung cancer patients.
方法以CK-19为标记,采用巢式RT-PCR和半定量方法对肺癌患者外周血中微转移灶的检测,比较治疗前后外周血中癌细胞数的相对变化。
补充资料：悬巢
1.登上巢车望敌。谓敌军压境。语本《左传．成公十六年》:"晋楚遇于鄢陵……楚子登巢车以望晋军。"陆德明释文:"兵车高如巢，以望敌也。"
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条