1)  DNA extraction
巢式PCR扩增
2)  IIFA
巢式
3)  nido configuration
巢式构型
4)  nested PCR
巢式PCR
1.
Amplify of fetal SRY gene in maternal plasma by using nested PCR;
应用巢式PCR扩增孕妇血浆中胎儿SRY基因
2.
Cloning of N-terminal deleted IκBα gene by nested PCR;
应用巢式PCR法克隆N端部分缺失的IκBα基因
3.
Establishment of a nested PCR assay for detection of Mycoplasma ovis;
羊附红细胞体巢式PCR检测方法的建立及临床应用
5)  hierarchical structure
巢式结构
1.
The experimental design to estimate genetic variances was hierarchical structure design via the natural selfing and was extended to advanced generations in order to get more equatio.
对F5世代68个家系的第7周株高、开花时株高资料和F6世代128个家系的最后株高资料进行三级、四级巢式结构的方差分析,得到足够的参数估计方程,在二级统计量水平上迭代估计各性状的主要遗传参数并计算狭义遗传力。
6)  Nested RT-PCR
巢式RT-PCR
1.
To study the incidence, the types of fusion genes and the clinical significance of rearrangements of mixed lineage leukemia (MLL) gene in acute leukemia (AL), the rearrangements of MLL gene of 60 patients with AL were detected by fluorescence in situ hybridization (FISH) and 6 types of common fusion genes resulting from the rearrangements of MLL gene were detected by nested RT-PCR.
为了研究混合系白血病(MLL)基因重排在急性白血病(AL)中的发生率、融合基因类型及其临床意义,用荧光原位杂交技术检测60例急性白血病(AL)患者MLL基因重排,对于MLL基因重排阳性的患者,用巢式RT-PCR方法检测MLL基因重排形成的6种常见融合基因类型。
2.
Methods CK19mRNA expression was detected by nested RT-PCR in the peripheral blood from 60 lung cancer patients.
方法以CK-19为标记,采用巢式RT-PCR和半定量方法对肺癌患者外周血中微转移灶的检测,比较治疗前后外周血中癌细胞数的相对变化。
3.
Immunohistochemical stain and nested RT-PCR were carried out to analysis the current status of Hepatitis E virus (HEV) infection.
用抗-人戊型肝炎病毒(HEV)抗体做肝脏中HEV抗原的免疫组织化学染色,用针对HEVORF2区设计的通用引物进行巢式RT-PCR,结果发现,6个地区581头屠宰猪的肝脏HEV免疫组织化学阳性率都在90%以上。
参考词条
补充资料:分子扩增
分子式:
CAS号:

性质:又称无性繁殖,克隆繁殖,分子扩增。通常是指用人工方法把所需要的某一供体生物的遗传物质,即DNA大分子提取出来,在离体条件下进行切割后,取其片段,或直接用人工合成的基因(DNA)在体外利用某些工具酶(限制性内切酶等)与作为载体的DNA分子(如质粒等)连接起来,然后再导入某一受体细胞中,让该外来遗传物质在其中进行正常复制和表达的过程。自20世纪70年代始,它是基因工程(gene engineering)、或微生物育种等领域中经常采用的方法。也是重要的克隆技术之一。

说明:补充资料仅用于学习参考,请勿用于其它任何用途。