1)  RT-PCR
“半巢式”PCR
2)  Semi-nested PCR
半巢式PCR
1.
Research on Promoter Region Methylation Status of Tumor Repressor Gene P16 in Human Hepatocellular Carcinoma by Using Methylation Sensitive Restriction Enzyme and Semi-nested PCR Assay;
限制性内切酶结合半巢式PCR法检测人肝癌P16抑癌基因启动子区甲基化研究
2.
A comparison of the detection sensitivity of five primer pairs(fOI1/rOI2c,fOI2/r23S1,fA2/rJ5,fP535/rP535,fP400/rP400)for citrus huanglongbing pathogen was carried out using simple PCR,semi-nested PCR and nested PCR.
半巢式PCR及巢式PCR的检测灵敏度远高于常规PCR,可检测出接近极限浓度10-7ng/μL,二者没有明显的差别。
3.
Semi-nested PCR method was established to detect trace ingredient of transgenic rapeseeds,which was 100 times more sensitive than the regular PCR.
建立了痕量转基因油菜籽成分的半巢式PCR(Semi-nested PCR)检测方法,灵敏度比常规PCR提高了100倍。
3)  semi-nested polymerase chain reaction
半巢式PCR
1.
we detected for HPV16DNA in 152 cases of cervical carcinoma and 60 cases of normal cervical epithelia with semi-nested polymerase chain reaction.
采用半巢式PCR技术对152例维吾尔族妇女宫颈癌石蜡组织中HPV16DNA进行检测。
2.
To investigate the correlation between HPV16 infection and p53 protein expression in cervical carcinoma among Uigur women in Xinjiang,we detected for HPV16DNA and p53 protein in 80 cases of cervical carcinoma and 34 cases of normal cervical epithelia with semi-nested polymerase chain reaction and immunohistochemistry methods,respectively.
采用半巢式PCR法检测 80例新疆维吾尔族宫颈癌及 3 4例正常宫颈组织中HPV16DNA ,采用免疫组化法检测p5 3蛋白 ,分析其与宫颈癌发生的相关性 ,探讨HPV16感染和p5 3蛋白表达在新疆维吾尔族妇女宫颈癌发生中的作用。
4)  nested PCR and semi-nested PCR
（半）巢式PCR
5)  cassette ligation semi-nested PCR
盒式连接半巢式PCR
6)  ban(半)
半
1.
The numerical characteristics of coefficient word " ban(半)" decide the mixing regularities have the different characteristics comparing with other coefficient words.
系数词"半"的数值特点决定了它与量词搭配规律与其它系数词相比具有不同的特点。
参考词条
补充资料：immune PCR
分子式：
CAS号：

性质： 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上，从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上，进而证明有抗原存在。目前最为敏感的检测方法，理论上可测得一个抗原分子的存在。

说明：补充资料仅用于学习参考，请勿用于其它任何用途。