1)  nested RT-PCR
巢式 RT-PCR
2)  Nested RT-PCR
巢式RT-PCR
1.
To study the incidence, the types of fusion genes and the clinical significance of rearrangements of mixed lineage leukemia (MLL) gene in acute leukemia (AL), the rearrangements of MLL gene of 60 patients with AL were detected by fluorescence in situ hybridization (FISH) and 6 types of common fusion genes resulting from the rearrangements of MLL gene were detected by nested RT-PCR.
为了研究混合系白血病(MLL)基因重排在急性白血病(AL)中的发生率、融合基因类型及其临床意义,用荧光原位杂交技术检测60例急性白血病(AL)患者MLL基因重排,对于MLL基因重排阳性的患者,用巢式RT-PCR方法检测MLL基因重排形成的6种常见融合基因类型。
2.
Methods CK19mRNA expression was detected by nested RT-PCR in the peripheral blood from 60 lung cancer patients.
方法以CK-19为标记,采用巢式RT-PCR和半定量方法对肺癌患者外周血中微转移灶的检测,比较治疗前后外周血中癌细胞数的相对变化。
3.
Immunohistochemical stain and nested RT-PCR were carried out to analysis the current status of Hepatitis E virus (HEV) infection.
用抗-人戊型肝炎病毒(HEV)抗体做肝脏中HEV抗原的免疫组织化学染色,用针对HEVORF2区设计的通用引物进行巢式RT-PCR,结果发现,6个地区581头屠宰猪的肝脏HEV免疫组织化学阳性率都在90%以上。
3)  semi-nested RT-PCR
半巢式RT-PCR
1.
Detection of respiratory syncytial virus by semi-nested RT-PCR;
半巢式RT-PCR法检测呼吸道合胞病毒
2.
Detection of Tobacco ringspot virus and Tomato ringspot virus by semi-nested RT-PCR;
烟草环斑病毒和番茄环斑病毒的半巢式RT-PCR检测
3.
Detection of Bean pod mottle virus by semi-nested RT-PCR in imported soybean;
半巢式RT-PCR检测进口大豆中菜豆荚斑驳病毒的研究
4)  Single cell nested RT-PCR
单细胞巢式RT-PCR
5)  Immuno-capture nested RT-PCR
免疫捕获巢式RT-PCR
6)  FQ-nRT-PCR
巢式荧光定量RT-PCR
参考词条
补充资料:法国mo-120-rt-61式120毫米迫击炮
Image:1159275166712025.jpg
法国mo-120-rt-61式120毫米迫击炮

rt-61迫击炮于1954年开始生产。该炮射程远、精度高、威力大、重量轻,而且具有鲜明的特色-线膛迫击炮,其身管外部还有螺旋纹,不仅可以用来精确调整火炮射角,同时还能增加身管的散热面积。火炮发射榴弹时最大射程为8135米,发射火箭增程弹时达13000米,最大射速20发/分,正常射速6发/分。全炮空重582千克,炮身长2080毫米。该炮的缺点是结构较为复杂,且不能直接瞄准,但瑕不掩瑜,现已有多个国家将其改进为自行迫击炮。

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