1) nested PCR-RFLP
巢式PCR-RFLP
1.
he differentiation of Cryptosporidium parvum,C.andersoni,and C.meleagrides by Nested PCR-RFLP analysis of the SSU rRNA gene;
应用巢式PCR-RFLP方法鉴别微小隐孢子虫、安氏隐孢子虫和火鸡隐孢子虫的研究
2.
Isolation of two strains of Cryptosporidium from bovines by 18S rRNA gene nested PCR-RFLP
18Sr RNA基因巢式PCR-RFLP分析两株牛源隐孢子虫分离株
3) nested PCR/RFLP
套式PCR/RFLP
4) PCR RFLP
PCR-RFLP
1.
Twenty two Bradyrhizobium peanut strains isolated from the root nodules of peanut growing at four different sites in Sichuan province,southwest China,were characterized by intrinsic antibiotic resistance (IAR),randomly amplified polymorphic DNAs(RAPDs)and 16S 23S rDNA PCR RFLP to investigate the genomic diversity.
用IAR、RAPDs和16S-23SPCR-RFLP3种方法对采集自四川省4个不同地点的22株慢生型花生根瘤菌的遗传多样性进行了研究。
5) nested PCR
巢式PCR
1.
Amplify of fetal SRY gene in maternal plasma by using nested PCR;
应用巢式PCR扩增孕妇血浆中胎儿SRY基因
2.
Cloning of N-terminal deleted IκBα gene by nested PCR;
应用巢式PCR法克隆N端部分缺失的IκBα基因
3.
Establishment of a nested PCR assay for detection of Mycoplasma ovis;
羊附红细胞体巢式PCR检测方法的建立及临床应用
6) Nested RT-PCR
巢式RT-PCR
1.
Development of nested RT-PCR for detection animal rabies virus
动物狂犬病病毒巢式RT-PCR检测方法的建立
2.
To study the incidence, the types of fusion genes and the clinical significance of rearrangements of mixed lineage leukemia (MLL) gene in acute leukemia (AL), the rearrangements of MLL gene of 60 patients with AL were detected by fluorescence in situ hybridization (FISH) and 6 types of common fusion genes resulting from the rearrangements of MLL gene were detected by nested RT-PCR.
为了研究混合系白血病(MLL)基因重排在急性白血病(AL)中的发生率、融合基因类型及其临床意义,用荧光原位杂交技术检测60例急性白血病(AL)患者MLL基因重排,对于MLL基因重排阳性的患者,用巢式RT-PCR方法检测MLL基因重排形成的6种常见融合基因类型。
3.
Methods CK19mRNA expression was detected by nested RT-PCR in the peripheral blood from 60 lung cancer patients.
方法以CK-19为标记,采用巢式RT-PCR和半定量方法对肺癌患者外周血中微转移灶的检测,比较治疗前后外周血中癌细胞数的相对变化。
补充资料：RFLP
分子式：
CAS号：
性质：又称限制片长多态性。每一种基因型的DNA对某些限制性内切酶有固定的酶切位点，经酶作用后，在凝胶电泳图谱上产生固定的片段图谱。如基因型发生变化，则酶切位点或数目可能有差异，则产生的电泳图谱会有差异。这方法常用于遗传基因型的检查或病理检查。一般来讲高等生物个体都有特定的RFLP。
CAS号：
性质：又称限制片长多态性。每一种基因型的DNA对某些限制性内切酶有固定的酶切位点，经酶作用后，在凝胶电泳图谱上产生固定的片段图谱。如基因型发生变化，则酶切位点或数目可能有差异，则产生的电泳图谱会有差异。这方法常用于遗传基因型的检查或病理检查。一般来讲高等生物个体都有特定的RFLP。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条