实时定量RT-PCR,real-time RT-PCR,在线英语词典,英文翻译,专业英语

1) real-time RT-PCR

实时定量RT-PCR

The viruses from concentrated samples were detected by real-time RT-PCR.

向水样中接种已知量的脊髓灰质炎病毒,然后利用微孔滤膜吸附—洗脱法、滑石粉—硅藻土吸附层吸附—洗脱法、化学絮凝沉淀法和滑石粉—硅藻土絮凝沉淀法等4种不同的浓缩方法对水样中的病毒进行浓缩,并采用实时定量RT-PCR技术对各方法浓缩后样品中的病毒进行扩增和检测。

By means of real-time RT-PCR,microporous filters with various nominal pores and materials were compared;the membrane elution method was modified;the effect of PEG on virus recovery was studied;the optimal method was determined at last.

通过实时定量RT-PCR检测,比较了不同材料和不同孔径的微孔滤膜对病毒的吸附效果;对膜洗脱方式进行了改进;研究了在洗脱液浓缩过程中,PEG浓度对于病毒回收率的影响。

The pattern of murine endogenous retrovirus-like gene(MuERV-L)mRNA expression was examined in the early preimplantation development of mice,using real-time RT-PCR technique.

利用实时定量RT-PCR技术,对体外培养的小鼠植入前胚胎发育过程中鼠内源逆转录病毒样基因(MuERV-L)的表达进行研究,并通过appd icolin(DNA复制抑制剂)、TSA(组蛋白去乙酰化酶特异性抑制剂)分别抑制1-细胞期DNA的复制和合子基因组激活(ZGA)过程中组蛋白去乙酰化,探索MuERV-L基因表达的变化。

2) real-time RT-PCR

real-time RT-PCR

Development of a TaqMan MGB Real-time RT-PCR aassay for West Nile virus detection;

TaqMan MGB Real-time RT-PCR检测西尼罗病毒方法的建立

Detection on Different Resistance of Two Rice Varieties Against Rice stripe virus in Real-Time RT-PCR;

应用Real-Time RT-PCR鉴定2个水稻品种(品系)对水稻条纹病毒的抗性差异

In order to control its epidemic,we designed the specific primers and probe following large scale norovirus genome consensus analysis and subsequently established a TaqMan-based Real-time RT-PCR assay for detection of genogroupⅡ(GGⅡ) norovirus RNA.

为加强其控制,通过序列比对设计出针对GGⅡ型诺如病毒保守序列的特异性引物与探针,建立了TaqMan Real-time RT-PCR方法。

3) real-time RT-PCR

实时荧光RT-PCR

Development and application of a real-time RT-PCR assay for fish viral nervous necrosis virus;

鱼类神经坏死病毒实时荧光RT-PCR检测方法的建立和应用

The Establishment of a Real-time RT-PCR Assay for Detection of Hepatitis E Virus (HEV) and Its Primary Application;

戊型肝炎病毒（HEV）实时荧光RT-PCR检测方法的建立及其初步应用

Methods:Using EVA Green,real-time RT-PCR methods for the detection of BCR/ABL,FoxO3a and GAPDH were explored.

方法:利用EVA Green建立实时荧光RT-PCR检测BCR/ABL、FoxO3a和GAPDH的方法,检测25例CML初发和25例CML急变患者的外周血样本中BCR/ABL融合基因和FoxO3a的表达,以及30例正常人外周血样本中FoxO3a的表达,以GAPDH为内参基因,各组基因表达水平的差异采用△△CT相对定量法。

4) real-time RT-PCR

实时逆转录聚合酶链反应

5) real-time RT-PCR

实时定量RTPCR

6) real-time RT-PCR

荧光逆转录-聚合酶链反应

参考词条

Real time RT-PCR real time RT-PCR Real-time RT-PCR Real Time RT-PCR Real time RT-PCR Real-time RT-PCR Real-time RT-PCR Real time RT PCR Real-Time RT-PCR real time RT-PCR Real-time RT-PCR Pvs48

补充资料：immune PCR

分子式：
CAS号：

性质：通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上，从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上，进而证明有抗原存在。目前最为敏感的检测方法，理论上可测得一个抗原分子的存在。

说明：补充资料仅用于学习参考，请勿用于其它任何用途。

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1) real-time RT-PCR 实时定量RT-PCR 1. The viruses from concentrated samples were detected by real-time RT-PCR. 向水样中接种已知量的脊髓灰质炎病毒,然后利用微孔滤膜吸附—洗脱法、滑石粉—硅藻土吸附层吸附—洗脱法、化学絮凝沉淀法和滑石粉—硅藻土絮凝沉淀法等4种不同的浓缩方法对水样中的病毒进行浓缩,并采用实时定量RT-PCR技术对各方法浓缩后样品中的病毒进行扩增和检测。 2. By means of real-time RT-PCR,microporous filters with various nominal pores and materials were compared;the membrane elution method was modified;the effect of PEG on virus recovery was studied;the optimal method was determined at last. 通过实时定量RT-PCR检测,比较了不同材料和不同孔径的微孔滤膜对病毒的吸附效果;对膜洗脱方式进行了改进;研究了在洗脱液浓缩过程中,PEG浓度对于病毒回收率的影响。 3. The pattern of murine endogenous retrovirus-like gene(MuERV-L)mRNA expression was examined in the early preimplantation development of mice,using real-time RT-PCR technique. 利用实时定量RT-PCR技术,对体外培养的小鼠植入前胚胎发育过程中鼠内源逆转录病毒样基因(MuERV-L)的表达进行研究,并通过appd icolin(DNA复制抑制剂)、TSA(组蛋白去乙酰化酶特异性抑制剂)分别抑制1-细胞期DNA的复制和合子基因组激活(ZGA)过程中组蛋白去乙酰化,探索MuERV-L基因表达的变化。 2) real-time RT-PCR real-time RT-PCR 1. Development of a TaqMan MGB Real-time RT-PCR aassay for West Nile virus detection; TaqMan MGB Real-time RT-PCR检测西尼罗病毒方法的建立 2. Detection on Different Resistance of Two Rice Varieties Against Rice stripe virus in Real-Time RT-PCR; 应用Real-Time RT-PCR鉴定2个水稻品种(品系)对水稻条纹病毒的抗性差异 3. In order to control its epidemic,we designed the specific primers and probe following large scale norovirus genome consensus analysis and subsequently established a TaqMan-based Real-time RT-PCR assay for detection of genogroupⅡ(GGⅡ) norovirus RNA. 为加强其控制,通过序列比对设计出针对GGⅡ型诺如病毒保守序列的特异性引物与探针,建立了TaqMan Real-time RT-PCR方法。 3) real-time RT-PCR 实时荧光RT-PCR 1. Development and application of a real-time RT-PCR assay for fish viral nervous necrosis virus; 鱼类神经坏死病毒实时荧光RT-PCR检测方法的建立和应用 2. The Establishment of a Real-time RT-PCR Assay for Detection of Hepatitis E Virus (HEV) and Its Primary Application; 戊型肝炎病毒（HEV）实时荧光RT-PCR检测方法的建立及其初步应用 3. Methods:Using EVA Green,real-time RT-PCR methods for the detection of BCR/ABL,FoxO3a and GAPDH were explored. 方法:利用EVA Green建立实时荧光RT-PCR检测BCR/ABL、FoxO3a和GAPDH的方法,检测25例CML初发和25例CML急变患者的外周血样本中BCR/ABL融合基因和FoxO3a的表达,以及30例正常人外周血样本中FoxO3a的表达,以GAPDH为内参基因,各组基因表达水平的差异采用△△CT相对定量法。 4) real-time RT-PCR 实时逆转录聚合酶链反应 5) real-time RT-PCR 实时定量RTPCR 6) real-time RT-PCR 荧光逆转录-聚合酶链反应参考词条 Real time RT-PCR real time RT-PCR Real-time RT-PCR Real Time RT-PCR Real time RT-PCR Real-time RT-PCR Real-time RT-PCR Real time RT PCR Real-Time RT-PCR real time RT-PCR Real-time RT-PCR Pvs48 补充资料：immune PCR 分子式： CAS号：性质：通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上，从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上，进而证明有抗原存在。目前最为敏感的检测方法，理论上可测得一个抗原分子的存在。说明：补充资料仅用于学习参考，请勿用于其它任何用途。
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