1) RT-PCR
RT-PCR
1.
Study on the Detection of Hepatitis a Virus in Shellfish by RT-PCR;
RT-PCR法检测贝类中的甲肝病毒的研究
2.
AMPLIFICATION AND VERIFICATION OF I-Eβ GENE OF MUS MUSCULUS THROUGH RT-PCR AND SIGNIFICANCE;
以RT-PCR技术扩增并鉴定小鼠I-Eβ基因及其意义
3.
Immunobeads combine RT-PCR detection of gastric cancer tumor markers α 4GnT;
免疫磁珠联合RT-PCR检测胃癌肿瘤标志物α4GnT
2) RT-PCR
逆转录聚合酶链反应
1.
METHODS: The Balb/c mice were treated with saline, BCG-PSN and lipopolysaccharide(LPS) respectively, and mIFNγ cDNA was amplified by RT-PCR.
方法:分别采用生理盐水、卡介菌多糖核酸和脂多糖处理Balb/c小鼠,逆转录聚合酶链反应(RT-PCR)技术扩增编码小鼠干扰素γ(mIFNγ)cDNA,通过RT-PCR产物来评价卡介菌多糖核酸诱导mIFNγ表达的能力,并设β-actin为对照。
2.
RT-PCR was performed for the hTERT mRNA in urine exfoliated cells.
方法采用逆转录聚合酶链反应(reverse transcriptase PCR,RT-PCR)检测42例膀胱癌、38例膀胱良性疾病患者和12例健康体检者晨尿中脱落细胞hTERT mRNA的表达,并与尿脱落细胞学检查结果比较;随访观察其中37例膀胱癌术后尿液hTERT mRNA变化与复发的关系。
3.
Methods Two siRNAs against the VEGF gene were designed and transfected into T24 cells respectively,VEGF gene expression was measured using RT-PCR.
方法体外构建两个针对VEGF基因的siVEGF的重组质粒,转染T24细胞;应用逆转录聚合酶链反应(RT-PCR)测定VEGF基因的表达,并用MTT法和流式细胞仪检测siVEGF对细胞的增殖抑制率和凋亡的影响。
3) RT-PCR
逆转录-聚合酶链反应
1.
Methods:Reverse transcription polymerase chain reaction(RT-PCR) was used to detect the expression of RASSF1A mRNA in 29 cases of human transitional cell carcinoma of bladder and 5 cases of normal bladder tissues.
方法:采用逆转录-聚合酶链反应(RT-PCR)检测29例膀胱移行细胞癌组织及5例正常膀胱组织中RASSF1A mRNA的表达水平。
2.
Results:RT-PCR and the sequence analysis of its product suggested that these 58 strains of measles viruses should belong to the H1 genotype of the eighth genetic group of wild measles virus.
结果:该58株病毒经逆转录-聚合酶链反应和其产物的基因序列分析显示,这58株病毒属于麻疹野病毒第8基因组H1基因型。
3.
Methords: The expression of PCA-1 mRNA was detected by RT-PCR in the samples from 45 cases of PCa with various clinico-pathologic characteristics, 30 cases of high-grade prostatic intraepithelial neoplasia (HG-PIN), 43 cases of BPH and 39 cases of other carcinorma tissues.
方法:采用逆转录-聚合酶链反应(RT-PCR)技术,检测45例PCa组织、30例前列腺高分级上皮样内瘤样病变组织(HG-PIN)、43例BPH组织和39例其他肿瘤组织标本中PCA-1 mRNA的表达。
4) RT-PCR
RTPCR
1.
Methods:Total RNA as well as mRNA were isolated from splenic cells of Balb/c mice and the entire coding cDNA sequence of GATA3 was amplified by reverse transcription polymerase chain reaction(RT-PCR).
方法:利用RTPCR方法,从Balb/c小鼠脾细胞的mRNA中扩增GATA3基因全长cDNA,并将其连接到pMD18-T载体上,进行测序和核酸分析。
2.
In present study, atherosclerotic animal model were replicated with high cholesterol feedstuff , and the expressions of estrogen receptor α and low-density lipoprotein receptor mRNA in heart and liver were studied by half quantitive RT-PCR, the rabbits oophorectomized and fed with normal feedstuff were used as control.
采用βactin做内参照的半定量RTPCR方法,对2组去势雌兔心脏、肝脏组织内雌激素受体α和低密度脂蛋白受体mRNA的表达进行了研究。
3.
Based on epidemical investigation, clinical symptom and pathology changes, both RT-PCR and PCR were used to detect porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type-2 (PCV-2) respectively.
在流行病学调查、临床症状、病理剖检的基础上,采用已建立的诊断猪繁殖与呼吸综合征病毒的RTPCR技术和诊断猪圆环病毒2型的PCR技术,从这几个规模化猪场的病料中均扩增出特异的目的基因片段,证实为猪繁殖与呼吸综合征病毒和猪圆环病毒2型混合感染。
5) RT-PCR
反转录-聚合酶链反应
1.
Studies on the Detection of Porcine Reproductive and Respiratory Syndrome Virus by RT-PCR;
用反转录-聚合酶链反应检测猪繁殖与呼吸综合征病毒的研究
2.
The expression of CGRP mRNA in trigeminal ganglion were detected with reverse transcription-polymerasa chain reaction(RT-PCR).
方法:采用反转录-聚合酶链反应(RT-PCR)技术检测正畸加力和撤力后不同时期大鼠三叉神经节CGRP mRNA水平的变化。
3.
Methods The mRNA expression of the survivin gene was evaluated by reverse transcription-polymerase chain reaction(RT-PCR) in 40 laryngeal carcinomas and in 52 non-neoplastic laryngeal tissues.
方法应用反转录-聚合酶链反应(RT-PCR)检测喉鳞癌组织、癌旁组织、非典型增生组织和正常对照组织中Survivin mRNA的表达水平。
6) RT-PCR
RT-PCR法
1.
The Detection of Norwalk Virus in Food by RT-PCR;
用RT-PCR法检测食品中诺沃克样病毒
2.
Clinical significance of detecting plasma HIV RNA concentration by RT-PCR;
RT-PCR法检测血浆HIV RNA浓度的临床意义
3.
Method To gather 500 blood serum samples of patients (diagnosis undetermined, DU), apply restriction enzyme analysis,Elisa, RT-PCR analysis to detect samples three times, and contrast the results of each method.
方法收集500例疑似病毒性心肌炎的患者血清,分别采用限制性内切酶分析法、ELISA法、RT-PCR法3种检测方法进行检测,每种方法在相同条件下连续重复3次,然后将其结果进行比较。
补充资料：immune PCR
分子式：
CAS号：
性质： 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上，从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上，进而证明有抗原存在。目前最为敏感的检测方法，理论上可测得一个抗原分子的存在。
CAS号：
性质： 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上，从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上，进而证明有抗原存在。目前最为敏感的检测方法，理论上可测得一个抗原分子的存在。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。