1) Real-time RT-PCR
实时RT-PCR
1.
Detection of Venezuelan equine encephalitis virus in different tissue samples of infected mice by real-time RT-PCR;
实时RT-PCR法检测感染小鼠不同组织标本中的委内瑞拉马脑炎病毒
2.
Establishment of the real-time RT-PCR assay for the detection of foot and mouth disease virus;
口蹄疫病毒实时RT-PCR检测方法的建立
3.
Objective To develop a simple and accurate method for the quantification of real-time RT-PCR gene products.
目的利用实时RT-PCR技术建立一种简便、准确的基因表达相对定量方法。
2) real time RT-PCR
实时RT-PCR
1.
To establish the real time RT-PCR method to measure the expression of c-erbB-2 mRNA in breast cancer tissues of beside tumors and tissues of adenosis of mammary glands, to find out the relationship of c-erbB-2 protein.
建立LightCycler实时RT-PCR方法检测乳腺癌、癌旁组织及乳腺腺病组织c-erbB-2基因mRNA的表达。
4) realtime PCR/RT-PCR
实时定量PCR/RT-PCR
5) real-time RT-PCR
实时定量RT-PCR
1.
The viruses from concentrated samples were detected by real-time RT-PCR.
向水样中接种已知量的脊髓灰质炎病毒,然后利用微孔滤膜吸附—洗脱法、滑石粉—硅藻土吸附层吸附—洗脱法、化学絮凝沉淀法和滑石粉—硅藻土絮凝沉淀法等4种不同的浓缩方法对水样中的病毒进行浓缩,并采用实时定量RT-PCR技术对各方法浓缩后样品中的病毒进行扩增和检测。
2.
By means of real-time RT-PCR,microporous filters with various nominal pores and materials were compared;the membrane elution method was modified;the effect of PEG on virus recovery was studied;the optimal method was determined at last.
通过实时定量RT-PCR检测,比较了不同材料和不同孔径的微孔滤膜对病毒的吸附效果;对膜洗脱方式进行了改进;研究了在洗脱液浓缩过程中,PEG浓度对于病毒回收率的影响。
3.
The pattern of murine endogenous retrovirus-like gene(MuERV-L)mRNA expression was examined in the early preimplantation development of mice,using real-time RT-PCR technique.
利用实时定量RT-PCR技术,对体外培养的小鼠植入前胚胎发育过程中鼠内源逆转录病毒样基因(MuERV-L)的表达进行研究,并通过appd icolin(DNA复制抑制剂)、TSA(组蛋白去乙酰化酶特异性抑制剂)分别抑制1-细胞期DNA的复制和合子基因组激活(ZGA)过程中组蛋白去乙酰化,探索MuERV-L基因表达的变化。
6) real-time quantitative RT-PCR
实时定量RT-PCR
1.
The clinical value of real-time quantitative RT-PCR detection for fusion genes in leukemia;
实时定量RT-PCR技术检测白血病融合基因的临床应用价值
2.
Development and application of Taqman real-time quantitative RT-PCR assay for detection of taura syndrome virus in shrimps;
对虾Taura综合症病毒Taqman实时定量RT-PCR检测方法的建立与应用
3.
And expressions of TLR9 mRNA on peripheral blood,were detected by using real-time quantitative RT-PCR method.
选取HSV阳性样品50例,选取HSV阴性10例做为对照组;采用实时定量RT-PCR方法进行TLR9 mRNA检测。
补充资料：immune PCR
分子式：
CAS号：
性质： 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上，从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上，进而证明有抗原存在。目前最为敏感的检测方法，理论上可测得一个抗原分子的存在。
CAS号：
性质： 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上，从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上，进而证明有抗原存在。目前最为敏感的检测方法，理论上可测得一个抗原分子的存在。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条