1) Multi RT-PCR
二联RT-PCR
1.
Establishment and Primary Application of Multi RT-PCR for Porcine Transmissible Gastroenteritis and Porcine Epidemic Diarrhoea;
猪传染性胃肠炎和猪流行性腹泻二联RT-PCR诊断方法的建立及初步应用
2) multiplex RT-PCR
多联RT-PCR
1.
The results showed thatthree specific bands,about 490 bp ,365 bp and 686 bp respectively,were amplified by multiplex RT-PCR of H5,H7 and H9 subtype of avian influenza virus with three sets of specific primers.
为快速、敏感、特异地鉴别诊断现较为重要的禽流感病毒亚型,根据H5、H7和H9亚型禽流感病毒HA基因序列的保守区域设计出并合成3对特异性引物,利用这3对引物对H5、H7和H9亚型禽流感病毒的HA基因片断进行多联RT-PCR扩增,并对多联RT-PCR检测方法进行敏感性及特异性实验。
2.
The results showed that three specific bands,about 385 bp,520 bp and 748 bp individually,were amplified by multiplex RT-PCR of AIV、NDV and IBV with t.
利用分子生物学方法,选择禽流感病毒的M基因、新城疫病毒的F基因以及传染性支气管炎病毒的N基因,分别在基因序列的保守区域设计1对特异性引物,利用所合成的3对引物对相应靶基因进行RT-PCR扩增,在优化单项RT-PCR条件的基础上,建立禽流感、新城疫、传染性支气管炎病毒多联RT-PCR的检测方法,同时对该检测方法进行敏感性及特异性试验。
3) duplex RT-PCR
二重RT-PCR
1.
Development and Application of A Duplex RT-PCR Method to Differentiate the PRRSV Variant and Common Strains
PRRSV变异株与普通株二重RT-PCR鉴别方法的建立与应用
2.
Developmemt of a duplex RT-PCR assay for detection of encephalomyocarditis virus and porcine reproductive and respiratory syndrome virus
猪脑心肌炎病毒和猪繁殖与呼吸综合征病毒二重RT-PCR检测方法的建立
4) two-temperature multiplex RT-PCR
二温式多重RT-PCR
1.
Development of two-temperature multiplex RT-PCR technique for detecting two viruses on orchid;
二温式多重RT-PCR同时检测两种主要兰花病毒的研究
5) one-step and two-temperature RT-PCR
一步二温式RT-PCR
6) reverse transcription-polymerase chain reaction
RT-PCR
1.
With beta-actin gene as the internal standard,reverse transcription-polymerase chain reaction(RT-PCR) was used to examine the expression of fms-like tyrosine kinase(Flt-1) mRNA in endometrium of Small Tail Han sheep and Northeast Fine-wool sheep.
以β-actin基因作为内源性内标,采用RT-PCR方法检测了Flt-1基因mRNA在东北细毛羊、小尾寒羊妊娠期不同阶段子宫内膜中的表达量。
2.
MethodsThe expression of FEZ1 mRNA in 50 cases of esophageal carcinoma and 50 cases of normal esophageal mucoma were detected by reverse transcription-polymerase chain reaction(RT-PCR).
方法采用RT-PCR法检测50例食管鳞癌及50例正常食管组织中FEZ1 mRNA的表达情况,分析其与食管鳞癌患者各临床病理指标的关系。
3.
Methods The mRNA of Ki-67 and VEGF were detected using reverse transcription-polymerase chain reaction(RT-PCR) in 55 RCC tissues and 20 normal renal tissues.
方法应用半定量RT-PCR技术检测55例肾癌组织和20例正常肾组织Ki-67和VEGFmRNA表达情况。
补充资料:1,2-联二乙基氨基甲酰二硫酸二钠
CAS:142-59-6
分子式:C4H8N2S4·2Na
中文名称:1,2-联二乙基氨基甲酰二硫酸二钠
英文名称:1,2-ethanediylbiscarbamodithioic acid disodium salt
1,2-ethanediylbis-carbamodithioic aci disodium salt
分子式:C4H8N2S4·2Na
中文名称:1,2-联二乙基氨基甲酰二硫酸二钠
英文名称:1,2-ethanediylbiscarbamodithioic acid disodium salt
1,2-ethanediylbis-carbamodithioic aci disodium salt
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条