1) Multiplex Immuno-capture RT-PCR
多联免疫捕获RT-PCR
2) IC-RT-PCR
免疫捕获RT-PCR
1.
In this study,based on the previously reported viral coat protein(CP)gene sequences,specific primers for ORSV and CyMV were designed,and four methods,including ELISA,RT-PCR,nested RT-PCR and IC-RT-PCR were performed for molecular detection of the two viruses.
本研究根据病毒外壳蛋白基因设计特异性引物,应用ELISA、普通RT-PCR、巢式RT-PCR和免疫捕获RT-PCR4种方法进行了检测研究与比较。
3) Immuno-capture nested RT-PCR
免疫捕获巢式RT-PCR
5) Immunocapture Reverse transcription a
免疫捕获反转录聚合酶链式反应(IC-RT-PCR)
6) multiplex RT-PCR
多联RT-PCR
1.
The results showed thatthree specific bands,about 490 bp ,365 bp and 686 bp respectively,were amplified by multiplex RT-PCR of H5,H7 and H9 subtype of avian influenza virus with three sets of specific primers.
为快速、敏感、特异地鉴别诊断现较为重要的禽流感病毒亚型,根据H5、H7和H9亚型禽流感病毒HA基因序列的保守区域设计出并合成3对特异性引物,利用这3对引物对H5、H7和H9亚型禽流感病毒的HA基因片断进行多联RT-PCR扩增,并对多联RT-PCR检测方法进行敏感性及特异性实验。
2.
The results showed that three specific bands,about 385 bp,520 bp and 748 bp individually,were amplified by multiplex RT-PCR of AIV、NDV and IBV with t.
利用分子生物学方法,选择禽流感病毒的M基因、新城疫病毒的F基因以及传染性支气管炎病毒的N基因,分别在基因序列的保守区域设计1对特异性引物,利用所合成的3对引物对相应靶基因进行RT-PCR扩增,在优化单项RT-PCR条件的基础上,建立禽流感、新城疫、传染性支气管炎病毒多联RT-PCR的检测方法,同时对该检测方法进行敏感性及特异性试验。
补充资料：免疫学技术酶联免疫检测仪
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说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条