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1)  immublotting
蛋白印迹杂交
2)  Western blot
蛋白质印迹杂交
1.
Methods: The quantitative analysis of the protein content and the DNA binding activity of NF-kB was examined by the Western blot method and electrophoretic mobility shift assay(EMSA) method in 20 cases of laryngeal carcinoma tissues and 10 cases of normal pericarcinoma tissues.
方法:采用蛋白质印迹杂交法(Western blot法)和凝胶电泳迁移率(EMSA)法对20例喉癌组织和10例癌旁正常组织中NF-kB的蛋白含量以及NF-kB DNA结合活性进行定量检测,并进行分析比较。
3)  blot hybridization
印迹杂交
1.
A series of DNA and RNA were detected by dot blot hybridization analysis.
本文利用RT-PCR和PCR技术制备地高辛标记的谷胱甘肽过氧化物酶(ATGPX3)cDNA探针,分别进行DNA和RNA斑点和印迹杂交分析。
4)  Western blot
蛋白印迹
1.
Methods The immunohistochemistry SABC method and Western blot were used to determine the expression of Annexin A1 in 120 cases of normal breast tissues,ductal hyperplasia,non-invasive carcinoma and invasive carcinoma.
方法应用免疫组织化学SABC方法和蛋白印迹检测乳腺正常组织、良性病变(纤维腺瘤)、癌前病变(乳腺囊性增生症)、非浸润性导管癌、浸润性导管癌中Annexin A1的表达情况。
2.
The aliquot of proteins was subjected to SDS-PAGE,followed by Western blot analysis in which individual sera were tested for primary antibody.
用肺癌患者及健康对照者的血清作第一抗体进行蛋白印迹检测。
3.
Methods The immunohistochemical method and Western blot method were used to determine expression of COX-2 protein in specimens of 47 cases(33 cases by IHC and 14 by Western Blot)of nasopharyngeal carcinoma and 24 cases of normal nasopharyngitis tissues.
方法分别应用免疫组化方法(IHC)和蛋白印迹方法(Western Blot)检测47例鼻咽癌组织(其中用IHC33例,Western Blot14例)及24例鼻咽部炎症组织中COX-2蛋白的表达。
5)  Western blotting
蛋白印迹
1.
METHODS:PDGFRαand C-kit gene mutations and protein expression in 52 cases of GIST were detected by polymerase chain reaction single- strand conformation polymorphism,immuno- histochemistry and Western blotting.
方法:采用单链象多态性聚合酶链式反应(PCR-SSCP),免疫组化和蛋白印迹(Western blot)方法,检测GIST 52例中PDGFRα和C-kit基因突变及蛋白表达情况。
2.
Method:Western blotting technique was used to test 60 serum samples.
方法 : 用蛋白印迹技术检测与麦芽糖结合蛋白 (MBP)融合的 2种重组梅毒螺旋体蛋白 (rTP47和rTP17)与二期梅毒患者血清的反应性。
6)  Western-blot
蛋白印迹
1.
The levels of bcl-2 and bax expression in cardiomyocytes were measured by RT-PCR and Western-blot.
光镜下观察细胞形态;逆转录-多聚酶链反应和蛋白印迹法检测bc l-2、bax蛋白在心肌细胞中的表达情况。
补充资料:印迹杂交
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性质:在基因操作中,把DNA或RNA、蛋白质等在薄膜滤器上先经浸润,固定后,于薄膜滤器上进行杂交,生成杂种分子。为基因操作中最常用的技术。常用的操作方法是:将用凝胶电泳分离出来的DNA或RNA、蛋白质等,在保持电泳图形的条件下,使其移至薄膜滤器上,再用事先准备好的检测样品(探针,经标记的某种基因等)进行杂交。以显示这些DNA、RNA、蛋白质等。从凝胶上转移DNA的技术称萨瑟恩(Southern)印迹技术。一般把转移RNA称为诺森(Northern)印迹技术,而把转移蛋白质称为韦斯顿(Western)印迹技术,当利用凝胶进行抗原抗体反应,再进行印迹时,称做伊斯特尔(Eastern)印迹技术。

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