1) Western blot
蛋白印迹法
1.
M ethod:Using Western blot method to measure HSP of hypothalamus in febr ile and hypothermal rats.
方法 :取高、低体温大鼠下丘脑组织 ,应用蛋白印迹法测定热休克蛋白含量。
2.
Methods:Western blot was used to detect the expression of Smad3 protein in choriocarci- noma cell line JEG-3 cell that was stimulated by TGF-β_1 with different density(0ng/ml,100ng/ml and 200ng/ ml).
方法:以绒癌细胞系JEG-3为研究对象,分别用0ng/ml、100ng/ml及200ng/ml浓度的TGF-β1处理48h后,采用蛋白印迹法检测JEG-3细胞Smad3蛋白的表达。
3.
ERK1/2 and p-ERK1/2 were examined by Western blot and immunohisto-chemical methods in the esophageal squamous carcinoma and normal adjacent esophageal tissue (control).
方法：应用蛋白印迹法检测60例人食管鳞癌组织及癌旁正常食管粘膜组织中的ERK1／2、p-ERK1／2蛋白的表达情况。
2) Western blotting
蛋白印迹法
1.
Radioimmunoassay, RT-PCR and Western blotting techniques were used to evaluate the change of brain COX expression at different time points( before SNI, 1 h, 12 h, 1 d, 3 d, 7 d, 14 d, 30 d and 60 d after SNI); By exploring hot plate test, we observed the reacting time of animals after injection of saline, NS-398, SC-560 and ind.
对小鼠施行SNI后,我们采取放射免疫法,RT-PCR法和蛋白印迹法测定术前以及术后1h、12h、1d、3d、7d、14d、30d、60d等不同时间点的脑COX-1和COX-2的表达;采用热板法,分别测定小鼠在各时间点注射生理盐水、NS-398、SC-560或吲哚美辛后的疼痛阈值。
2.
Methods: By using Western blotting (WB), RPR test and TPPA, serologic antibodies were detected in 67 cases of syphilis, of whom 21 were followed up for 18 to 24 months after treatment.
方法:采用蛋白印迹法(Westernblotting,WB)、快速血浆反应素(RPR)试验、梅毒颗粒凝集试验(TPPA)3种方法,对67例梅毒及其中经治疗后随诊18～24个月的21例患者作血清检测。
3.
The techniques of diagnosis include ELISA and Western blotting.
细菌病原诊断技术包括酶联免疫吸附技术（ＥＬＩＳＡ）及蛋白印迹法（Ｗｅｓｔｅｒｎｂｌｏｔｉｎｇ）。
3) WB
蛋白印迹法
1.
Methods:Over a 5-year period(2000-2004),the serum antibodies measurement of against Lyme borreliosis with the serological tests:a sensitive IFA as the first step,followed by WB(IgM and IgG)in 2 167 people s serum specimen of Xinjiang natural foci compared with random sampling 1 443 people from out-patient clinic and hospital patients in country and ci.
方法:2000至2004年采用间接免疫荧光抗体法(IFA)、蛋白印迹法(WB),对业已证实的新疆自然疫源地区2167人份的血清标本进行抗LD螺旋体抗体(IgM、IgG)检测;采用IFA、WB、ELISA试验对来自21个县、市的门诊与住院患者,随机抽样1443人份血清标本进行抗LD螺旋体抗体(IgM、IgG)检测作对照;共计3610份血清标本。
2.
Methods:The sera of 123 suspected cases and part of spinal fluids were collected to detect the specific antibodies (IgM,IgG) to Borrdlia burgdorferi by enzyme linked immunosorbent assay (ELISA),indirect flouorescent antibody (IFA) test,and Western blot (WB).
方法:应用间接免疫荧光抗体法(IFA)、酶联免疫吸附试验(ELISA)、蛋白印迹法(WB)3种方法检测新疆123例疑似莱姆病患者血清及部分脑脊液抗伯氏疏螺旋体IgM、IgG抗体,其中3例PCR法检测尿液,结合相应的影像学、电生理检查诊断莱姆病。
4) western blotting
蛋白质印迹(法)，Western印迹法
5) Western Blot
免疫蛋白印迹法
1.
Methods Western blot and RT-PCR were employed to investigate the expression of hPR isoforms in ectopic lesion from adenomyosis and normal endometrium from controls.
方法运用免疫蛋白印迹法(Western blot)和RT-PCR方法测定子宫腺肌病患者(24例)病灶及其在位内膜及对照组(19例)子宫内膜标本中孕激素受体亚型在蛋白和mRNA水平的表达,计算机图像分析、半定量研究孕激素受体亚型的表达。
6) Western blotting
蛋白质印迹法
1.
), total RNA of livers were extracted and kinetics of the mRNA expression of iNOS were detected by RT-PCR, the protein expression of iNOS was then confirmed by Western blotting and the distribution of iNOS in the infected liver was determined by immunohistochemical .
方法 日本血吸虫尾蚴经皮肤感染NMRI小鼠 ,感染后第 0、2 1、2 8、3 8和 45天取其肝组织 ,用逆转录聚合酶链反应 (RT PCR)半定量法检测i NOS转录水平的动态表达 ,用蛋白质印迹法 (Westernblotting)测定表达的蛋白变化 ,并用免疫组化方法确定iNOS在肝组织中的表达定位。
补充资料：蛋白印迹法
分子式：
CAS号：
性质：一种类似于DNA斑迹法，用于从凝胶中转移蛋白质的技术。转移到膜上的蛋白质用同位素或酶标记过的抗体显示目的带的存在。
CAS号：
性质：一种类似于DNA斑迹法，用于从凝胶中转移蛋白质的技术。转移到膜上的蛋白质用同位素或酶标记过的抗体显示目的带的存在。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条