Northern印迹杂交,Northern blot,音标,读音,翻译,英文例句,英语词典

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1) Northern blot 点击朗读

Northern印迹杂交

Methods The probes corresponding to the genes were amplified by RT PCR from mouse kidney, and then Northern blotting was performed on mouse multiple tissues membrane.

方法RT-PCR方法扩增WNK1和WNK4基因片段作为探针,在小鼠多组织膜上进行Northern印迹杂交。

Method The specific fragments of the two transcripts were amplified by RT-PCR and identified by Northern blot.

方法　RT PCR扩增两个转录本的特异性片段 ,Northern印迹杂交证实片段特异性后 ,将片段克隆入pGEM T载体中 ,体外转录同位素标记的正义和反义RNA探针 ,在小鼠肾脏组织石蜡切片上进行原位杂交检测。

The gene expressions of matix metalloproteinase\|2(MMP\|2) and membrane type matrix metalloproteinase (MT\|MMP) and TIMP 1 were investigated by Northern blot,The gelatinase activity of MMP\|2 was exam.

方法 :以 0 5、2 0、4 0g kg等不同剂量抗纤复方灌胃大鼠制备的含药血清 ,作用HSC LI90细胞 4 8h ,应用Northern印迹杂交的方法 ,检测膜型基质金属蛋白酶、基质金属蛋白酶 2及其抑制因子 1(TIMP 1)基因的表达 ,并用酶图法检测基质金属蛋白酶 2的活性。

2) Northern blot analysis 点击朗读

Northern印迹杂交

Northern blot analysis and immunocytochemistry techniques were performed to detect the expression of typeⅠ,Ⅲ procollagen mRNA and their relevant proteins in cultured rat pulmonary fibrobasts(PFbs) after FN treatment.

方法　用免疫组织化学方法观察实验性大鼠肺纤维化纤维连接蛋白 (FN)的动态变化 ;用Northern印迹杂交和免疫细胞化学方法分别观察FN对体外培养的大鼠肺成纤维细胞Ⅰ、Ⅲ型前胶原mRNA和蛋白表达的影响。

3) Southen and Northen blotting 点击朗读

Southern和Northern印迹杂交技术

4) Northern blotting 点击朗读

Northern印迹

Methods DNA gel electrophoresis and Northern blotting hybridization were used to detect the apoptosis and the level of c myc gene,respectively.

方法　应用体外细胞培养法 ,DNA凝胶电泳技术、Northern印迹杂交技术。

更多例句>>

5) Northern blot 点击朗读

Northern印迹

MRP-1/CD9 gene expression in different kinds of gastric tissue was analyzed by Northern blot and reverse transc.

对其中的运动相关蛋白 (motility- related protein- 1,MRP- 1/CD9)基因 ,用 Northern印迹及逆转录 -聚合酶链反应技术分析在正常及不同病理状态下该基因表达的情况。

Among these techniques, Northern blot, ribonuclease protection assay(RPA), real-time quantitative polymerase chain reaction(RQ-PCR), which are based on differentially screening and hybridization technology, have been applied most frequently.

本文概述了用于检测mRNA丰度的常规分析方法,即Northern印迹、RNase保护试验和实时定量PCR技术,特别是为对数量不多的基因进行表达情况的测定时提供了较为实用的方法。

9 fold more in the resistant strain than in the susceptible strain, by reverse Northern blot and Northern blot assays.

用逆Northern印迹和Northern印迹法进一步鉴定淡色库蚊对溴氰菊酯抗药性和敏感性品系胰蛋白酶的表达差异 ,结果显示 ,胰蛋白酶基因在抗药性品系中的表达量分别是敏感性品系的 4。

更多例句>>

6) blot hybridization 点击朗读

印迹杂交

A series of DNA and RNA were detected by dot blot hybridization analysis. 点击朗读

本文利用RT-PCR和PCR技术制备地高辛标记的谷胱甘肽过氧化物酶(ATGPX3)cDNA探针,分别进行DNA和RNA斑点和印迹杂交分析。

更多例句>>

补充资料：印迹杂交

分子式：
CAS号：

性质：在基因操作中，把DNA或RNA、蛋白质等在薄膜滤器上先经浸润，固定后，于薄膜滤器上进行杂交，生成杂种分子。为基因操作中最常用的技术。常用的操作方法是：将用凝胶电泳分离出来的DNA或RNA、蛋白质等，在保持电泳图形的条件下，使其移至薄膜滤器上，再用事先准备好的检测样品(探针，经标记的某种基因等)进行杂交。以显示这些DNA、RNA、蛋白质等。从凝胶上转移DNA的技术称萨瑟恩(Southern)印迹技术。一般把转移RNA称为诺森(Northern)印迹技术，而把转移蛋白质称为韦斯顿(Western)印迹技术，当利用凝胶进行抗原抗体反应，再进行印迹时，称做伊斯特尔(Eastern)印迹技术。

说明：补充资料仅用于学习参考，请勿用于其它任何用途。

参考词条

Reverse Northern杂交 Northern印迹分析 Southern印迹杂交 Western印迹杂交 Northern杂交分析反向Northern杂交

Northern杂交 Northern点杂交

说明：双击或选中下面任意单词，将显示该词的音标、读音、翻译等；选中中文或多个词，将显示翻译。
	您的位置：首页 -> 词典 -> Northern印迹杂交 1) Northern blot Northern印迹杂交 1. Methods The probes corresponding to the genes were amplified by RT PCR from mouse kidney, and then Northern blotting was performed on mouse multiple tissues membrane. 方法RT-PCR方法扩增WNK1和WNK4基因片段作为探针,在小鼠多组织膜上进行Northern印迹杂交。 2. Method The specific fragments of the two transcripts were amplified by RT-PCR and identified by Northern blot. 方法　RT PCR扩增两个转录本的特异性片段 ,Northern印迹杂交证实片段特异性后 ,将片段克隆入pGEM T载体中 ,体外转录同位素标记的正义和反义RNA探针 ,在小鼠肾脏组织石蜡切片上进行原位杂交检测。 3. The gene expressions of matix metalloproteinase\\|2(MMP\\|2) and membrane type matrix metalloproteinase (MT\\|MMP) and TIMP 1 were investigated by Northern blot,The gelatinase activity of MMP\\|2 was exam. 方法 :以 0 5、2 0、4 0g kg等不同剂量抗纤复方灌胃大鼠制备的含药血清 ,作用HSC LI90细胞 4 8h ,应用Northern印迹杂交的方法 ,检测膜型基质金属蛋白酶、基质金属蛋白酶 2及其抑制因子 1(TIMP 1)基因的表达 ,并用酶图法检测基质金属蛋白酶 2的活性。 2) Northern blot analysis Northern印迹杂交 1. Northern blot analysis and immunocytochemistry techniques were performed to detect the expression of typeⅠ,Ⅲ procollagen mRNA and their relevant proteins in cultured rat pulmonary fibrobasts(PFbs) after FN treatment. 方法　用免疫组织化学方法观察实验性大鼠肺纤维化纤维连接蛋白 (FN)的动态变化 ;用Northern印迹杂交和免疫细胞化学方法分别观察FN对体外培养的大鼠肺成纤维细胞Ⅰ、Ⅲ型前胶原mRNA和蛋白表达的影响。 3) Southen and Northen blotting Southern和Northern印迹杂交技术 4) Northern blotting Northern印迹 1. Methods DNA gel electrophoresis and Northern blotting hybridization were used to detect the apoptosis and the level of c myc gene,respectively. 方法　应用体外细胞培养法 ,DNA凝胶电泳技术、Northern印迹杂交技术。更多例句>> 5) Northern blot Northern印迹 1. MRP-1/CD9 gene expression in different kinds of gastric tissue was analyzed by Northern blot and reverse transc. 对其中的运动相关蛋白 (motility- related protein- 1,MRP- 1/CD9)基因 ,用 Northern印迹及逆转录 -聚合酶链反应技术分析在正常及不同病理状态下该基因表达的情况。 2. Among these techniques, Northern blot, ribonuclease protection assay(RPA), real-time quantitative polymerase chain reaction(RQ-PCR), which are based on differentially screening and hybridization technology, have been applied most frequently. 本文概述了用于检测mRNA丰度的常规分析方法,即Northern印迹、RNase保护试验和实时定量PCR技术,特别是为对数量不多的基因进行表达情况的测定时提供了较为实用的方法。 3. 9 fold more in the resistant strain than in the susceptible strain, by reverse Northern blot and Northern blot assays. 用逆Northern印迹和Northern印迹法进一步鉴定淡色库蚊对溴氰菊酯抗药性和敏感性品系胰蛋白酶的表达差异 ,结果显示 ,胰蛋白酶基因在抗药性品系中的表达量分别是敏感性品系的 4。更多例句>> 6) blot hybridization 印迹杂交 1. A series of DNA and RNA were detected by dot blot hybridization analysis. 本文利用RT-PCR和PCR技术制备地高辛标记的谷胱甘肽过氧化物酶(ATGPX3)cDNA探针,分别进行DNA和RNA斑点和印迹杂交分析。更多例句>> 补充资料：印迹杂交分子式： CAS号：性质：在基因操作中，把DNA或RNA、蛋白质等在薄膜滤器上先经浸润，固定后，于薄膜滤器上进行杂交，生成杂种分子。为基因操作中最常用的技术。常用的操作方法是：将用凝胶电泳分离出来的DNA或RNA、蛋白质等，在保持电泳图形的条件下，使其移至薄膜滤器上，再用事先准备好的检测样品(探针，经标记的某种基因等)进行杂交。以显示这些DNA、RNA、蛋白质等。从凝胶上转移DNA的技术称萨瑟恩(Southern)印迹技术。一般把转移RNA称为诺森(Northern)印迹技术，而把转移蛋白质称为韦斯顿(Western)印迹技术，当利用凝胶进行抗原抗体反应，再进行印迹时，称做伊斯特尔(Eastern)印迹技术。说明：补充资料仅用于学习参考，请勿用于其它任何用途。参考词条 Reverse Northern杂交 Northern印迹分析 Southern印迹杂交 Western印迹杂交 Northern杂交分析反向Northern杂交

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