1) CTAB-LiCl-based extraction method
CTAB-LiCl提取法
1.
A novel and simple CTAB-LiCl-based extraction method for high-quality and total RNA of rice embryo samples is developed.
介绍了一种全新、简单的提取水稻种胚RNA的CTAB-LiCl提取法,可在不用液氮的室温下有效地排除剥取的种胚中大量带有的多糖和本身脂质的干扰,所得RNA样品经紫外分光光度计和琼脂糖凝胶电泳分析证明具有较高的纯度和完整性,并可进一步满足RT-PCR和Northern印迹的实验需要,适用于富含多糖和脂质的植物组织RNA的提取。
2) CTAB-LiCl method
CTAB-LiCl法
1.
The results show the higher yield and good quality of RNA are extracted using an improved CTAB-LiCl method which can be used directly in cDNA synthesis, cDNA-AFLP analysis and other molecular biological experiment.
试验以茶树种胚为研究对象,通过对几种提取总RNA方法的比较与分析,认为用改进的CTAB-LiCl法提取茶树种胚总RNA的产率较高,质量好,可直接用于cDNA的合成、cDNA-AFLP分析等分子生物学实验操作。
2.
Total RNA extracted by CTAB-LiCl method had high quality, which is the best method of total RNA isolation from Dioscorea opposita.
采用异硫氰酸胍-巯基乙醇联合变性法、Trizol法和CTAB-LiCl法等3种方法,分别对山药叶片和块茎进行总RNA提取效果进行了比较。
3) CTAB DNA method
CTAB DNA提取法
4) CTAB DNA extraction
CTAB法DNA提取
5) modified CTAB-LiCl
改良CTAB-LiCl法
1.
The results showed that the modified CTAB-LiCl was capable of efficiently removing polysaccharides,and that of the RNAs extracted by the modified CTAB-LiCl,the lightness of 28 s rRNA was two times as much as that of 18 S Rrna, the value of A260/A280,A260/A230 was1.
结果表明,改良CTAB-LiCl法能有效地去除多糖,提取到的RNA 28 SrRNA亮度约为18SrRNA的二倍,A260/A280比值为1。
6) CTAB miniprep
CTAB微量提取法
补充资料:licl
CAS:7447-41-8
分子式:ClLi
分子质量:42.39
中文名称:氯化锂;氯化锂(无水);氯化锂(结晶);氯化锂,无水
英文名称:Lithium chloride;Lithium chloride, anhydrous;chlorku litu;chlorure de lithium;licl;lithium chloride (licl);Lithiumchlorid
分子式:ClLi
分子质量:42.39
中文名称:氯化锂;氯化锂(无水);氯化锂(结晶);氯化锂,无水
英文名称:Lithium chloride;Lithium chloride, anhydrous;chlorku litu;chlorure de lithium;licl;lithium chloride (licl);Lithiumchlorid
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条