Three-dimensional structure modeling and functional analysis of 3D polymerase of foot-and-mouth disease virus strain AF72;

The 3D polymerase gene of Foot-and-mouth disease virus strain AF72 was amplified by reverse transcription polymerase chain reaction(RT-PCR),The purified PCR products were cloned into pGEM-T easy Vectors,and the nucleotide sequences were highly homologous to other four reference strains from GenBank.

coli DH5α was transformed with p Taq expressing plasmid within which the Taq DNA polymerase gene was inserted.

Novel Functional Block Copolymer Combining Enzymatic Polymerization and ATRP and Its Self-Assembly Behavior Study;

Gene deletion vector pXL05 was used to disrupt oligomycin polyketide synthase (PKS) encoding genes(olmA) in Streptomyces a.

The recombinant Sorangium cellulosum in which the epoK gene has been inactivated by random mutagenesis and so produces only epothilone D and C,does not produce epothilone A or B,duo to an alteration in the genes coding for the epothilone polyketide synthase(PKS).

OBJECTIVE To clone and characterize type Ⅲ polyketide synthases (PKSs) gene from Fallopia multiflora.

Subcloning and expression of influenza virus RNA polymerase PB1-1 fragment;

Isolation and purification of RNA polymerase from influenza virus;

Stringent RNA polymerase of E. coli and its in vivo transcriptional activity;