It could add proper adapter when the PCR primers were designed, and the purpose fragments were cloned by PCR, and the various complementary sticky ends were created by T4 DNA polymerase’s 3′-exodeoxyribonuclease activity.

coli DH5α was transformed with p Taq expressing plasmid within which the Taq DNA polymerase gene was inserted.

Novel Functional Block Copolymer Combining Enzymatic Polymerization and ATRP and Its Self-Assembly Behavior Study;

Gene deletion vector pXL05 was used to disrupt oligomycin polyketide synthase (PKS) encoding genes(olmA) in Streptomyces a.

The recombinant Sorangium cellulosum in which the epoK gene has been inactivated by random mutagenesis and so produces only epothilone D and C,does not produce epothilone A or B,duo to an alteration in the genes coding for the epothilone polyketide synthase(PKS).

OBJECTIVE To clone and characterize type Ⅲ polyketide synthases (PKSs) gene from Fallopia multiflora.

Subcloning and expression of influenza virus RNA polymerase PB1-1 fragment;

Isolation and purification of RNA polymerase from influenza virus;

Stringent RNA polymerase of E. coli and its in vivo transcriptional activity;

Screening and identification of genes transactivated by the functional domains of hepatitis B virus DNA polymerase using cDNA microarray;

The perioperative serum DNA polymerase activity changes in patients with gastric carcinoma;

The cloning of putative DNA polymerase from white spot bacilliform virus;