To improve the specificity of PCR in detection of genes in genomic DNA, a modified touchdown PCR method was designed by using the common Taq DNA polymerase and the Pfu DNA polymerase with proofreading ability.

coli DH5α was transformed with p Taq expressing plasmid within which the Taq DNA polymerase gene was inserted.

Novel Functional Block Copolymer Combining Enzymatic Polymerization and ATRP and Its Self-Assembly Behavior Study;

Gene deletion vector pXL05 was used to disrupt oligomycin polyketide synthase (PKS) encoding genes(olmA) in Streptomyces a.

The recombinant Sorangium cellulosum in which the epoK gene has been inactivated by random mutagenesis and so produces only epothilone D and C,does not produce epothilone A or B,duo to an alteration in the genes coding for the epothilone polyketide synthase(PKS).

OBJECTIVE To clone and characterize type Ⅲ polyketide synthases (PKSs) gene from Fallopia multiflora.

Subcloning and expression of influenza virus RNA polymerase PB1-1 fragment;

Isolation and purification of RNA polymerase from influenza virus;

Stringent RNA polymerase of E. coli and its in vivo transcriptional activity;

Screening and identification of genes transactivated by the functional domains of hepatitis B virus DNA polymerase using cDNA microarray;

The perioperative serum DNA polymerase activity changes in patients with gastric carcinoma;

The cloning of putative DNA polymerase from white spot bacilliform virus;