The binding of human DNA polymerase β to DNA template-primer duplex and single-stranded DNA have been studied by using surface plasmon resonance biosensor.

Study on DNA polymerase β gene mutation in human gastric cancer tissues;

Expression of DNA polymerase β induced by all-trans retinoic acid and dimethyl sulfoxide and change of the base excision repair in human esophageal cancer cells;

Effect of over-expressed DNA polymerase β on malignant degree of esophageal cancer EC9706 cells;

Methods 40 cases of nasopharyngeal carcinoma (NPC) tissues were collected to study DNA polβgene mutation with PT-PCR, PCR-SSCP and DNA sequence analyzing technique, the 40 cases of NPC were divided into three groups according to pathology differentiation,16 cases of grade Ⅰ(G1) ,14 cases of grade Ⅱ(G2) ,10 cases of grade Ⅲ(G3).

Western blotting and semi-quantitative RT-PCR were employed to analyze expression of the DNA polβ protein and DNA polβ mRNA,res.

Aim: To establish a cell line stably expressing mutant (58 bp deletion)DNA polymerase β(DNA polβ).

Mutation characteristics of DNA polymerase beta gene in human gastric carcinoma tissue;

Detection of DNA polymerase beta gene expression in human esophageal cancer tissues by RNA dot blotting;

Objectives: DNA polymerase beta plays an important role in repair of damaged DNA, especially in base excision repair (BER).

Vector-mediated RNA interference of DNA polymerase beta in human bronchial epithelial cells;