1)  4 5S RNA
4.5S RNA
2)  4. 5S RNA gene
4.5S RNA基因
3)  RNA
RNA
1.
Study on Influence of Technology Parameter on Extraction of RNA from Brewing Yeast by Strong Salt;
影响浓盐法提取啤酒酵母RNA的工艺参数研究
2.
Study on Extracting RNA from Brewing Yeast by Strong Salt Method;
浓盐法提取啤酒酵母中RNA的研究
3.
Extracting RNA from spent brewer's yeast by the new technology rare salt of high temperature of weak base;
高温弱碱稀盐法从啤酒废酵母中提取RNA
4)  RNAⅢ
RNAⅢ
1.
Transcription level of trap and RNAⅢ was detected by real time RT-PCR.
方法采用微量板半定量法检测表皮葡萄球菌生物膜表型,二维电泳和高灵敏度的基质辅助激光解吸附飞行时间质谱分析比较表皮葡萄球菌标准株蛋白质表达谱,实时定量逆转录PCR检测trap基因和RNAⅢ的转录水平,CLUSTAL X对表皮葡萄球菌trap基因与金黄色葡萄球菌序列进行分析,Mega软件构建进化树。
5)  Northern
RNA
1.
Methods Forty-eight samples from patients with ovarian tumor at different clinical stages and 8 from normal ovaries were examined for NM23-H1B mRNA expression by using RT-PCR, northern blot and in situ hybridization.
采用RT-PCR技术、RNA印迹(northern blot)法、原位杂交实验,检测NM23-H1B基因mRNA的表达。
2.
METHODS: The validity and reliability of the results gotten by serial analysis of gene expression method was verified by RT-PCR and Northern blot.
以管家基因磷酸甘油醛脱氢酶(GAPDH)和肌动蛋白(β-actin)的mRNA表达水平为对照,比较了在JT库中表达水平比在WY库中高8倍的JT8标签对应基因的表达。
3.
Methods: Total RNA was isolated from peripheral leukocytes of healthy men and women, AR mRNA was determined by RT PCR and Northern blot.
方法 :采用 RT- PCR和 Northern印迹方法检测健康成年人外周血白细胞 AR m RNA的表达。
6)  Total RNA
RNA
1.
Extraction and Yield of Total RNA in Cochleas of Normal Rats and Long-Term Sodium Salicylate Treated Rats;
正常及慢性水杨酸钠作用后大鼠耳蜗总RNA的提取和产量
2.
By using the modified CTAB method, total RNA was isolated from Camellia oleifera leaves rich in such secondary products as polyphones and polymeric carbohydrates, and then mRNA was isolated and purified through double selections using oligo(dT) cellulose.
 利用改良的CTAB法,从富含酚类糖类等次生物质油茶叶片中分离出总RNA,并从总RNA中,通过两次过Oligo(dT)柱分离纯化得到mRNA。
参考词条
补充资料:感染性RNA病原RNA
分子式:
CAS号:

性质:又称感染性RNA病原RNA;壳病毒,是一种和病毒(virus)相似的感染性颗粒。为无蛋白外壳的单链RNA,分子量1.1×105~1.3×105。它是比已知病毒都小的能在宿主细胞内自主复制的病原体之一。已知的近20种类病毒中,大部分已测得了一级结构,都是无蛋白外壳的共价闭合的单链环状RNA分子。在天然状态下类病毒RNA以高度碱基配对的棒状结构形式存在。最先是由T. O. Diener等人(1969)在马铃薯纤块茎病(potato spindle tuber disease)的病株上首先发现的,在电镜下可见到这RNA分子呈50nm长的杆状分子,共有359个碱基对,并证实是游离的RNA,为此正式命名为类病毒。它通常在宿主细胞核内,借助汁液传染,分子量75000~130000,比最小病毒还小80倍。后又相继在菊花矮缩病(chrysanthemum stunt)、菊花绿斑病(chrysanthenum chlorotic mottle)、柑橘剥皮病(citrus excortis)等患病植株中分离到低分子量的病原RNA。推测它也可能存在于其他植物、动物甚至人体内。绝大部分类病毒均具有共同的结构特征:(1)位于棒状结构中心有一个高度保守的序列;(2)靠近这一保守中心区的左侧有一个多聚嘌呤区;(3)棒状结构左侧序列保守性强,右侧变异性大。它可能是通过核苷酸序列或结构改变直接与寄主细胞相互作用、干扰细胞的代谢而致病。对类病毒的研究可能为揭示生命起源和进化、生命过程的实现等生命科学的重大理论问题作出贡献。

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