1) Single enzyme RT PCR
单酶法RTPCR
2) Method of RT-PCR
RTPCR方法
3) RT-PCR
RTPCR
1.
Methods:Total RNA as well as mRNA were isolated from splenic cells of Balb/c mice and the entire coding cDNA sequence of GATA3 was amplified by reverse transcription polymerase chain reaction(RT-PCR).
方法:利用RTPCR方法,从Balb/c小鼠脾细胞的mRNA中扩增GATA3基因全长cDNA,并将其连接到pMD18-T载体上,进行测序和核酸分析。
2.
In present study, atherosclerotic animal model were replicated with high cholesterol feedstuff , and the expressions of estrogen receptor α and low-density lipoprotein receptor mRNA in heart and liver were studied by half quantitive RT-PCR, the rabbits oophorectomized and fed with normal feedstuff were used as control.
采用βactin做内参照的半定量RTPCR方法,对2组去势雌兔心脏、肝脏组织内雌激素受体α和低密度脂蛋白受体mRNA的表达进行了研究。
3.
Based on epidemical investigation, clinical symptom and pathology changes, both RT-PCR and PCR were used to detect porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type-2 (PCV-2) respectively.
在流行病学调查、临床症状、病理剖检的基础上,采用已建立的诊断猪繁殖与呼吸综合征病毒的RTPCR技术和诊断猪圆环病毒2型的PCR技术,从这几个规模化猪场的病料中均扩增出特异的目的基因片段,证实为猪繁殖与呼吸综合征病毒和猪圆环病毒2型混合感染。
4) RT PCR
RTPCR
5) RT PCR
逆转录聚合酶链反应(RTPCR)
6) One enzyme digestion method
单酶消化法
补充资料：酒化酶、醇酶、发酵酶
分子式：
CAS号：
性质：又称酒化酶、醇酶、发酵酶。从酵母中分离而得的一系列酶。不耐热。它能催化酒精发酵反应，与糖酵解有关，如磷酸化酶、醛缩酶、葡萄糖磷酸变位酶、葡萄糖6-磷酸酶等。
CAS号：
性质：又称酒化酶、醇酶、发酵酶。从酵母中分离而得的一系列酶。不耐热。它能催化酒精发酵反应，与糖酵解有关，如磷酸化酶、醛缩酶、葡萄糖磷酸变位酶、葡萄糖6-磷酸酶等。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条