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1)  RT PCR of DsRNA
dsRNA的RTPCR
2)  RT-PCR
RTPCR
1.
Methods:Total RNA as well as mRNA were isolated from splenic cells of Balb/c mice and the entire coding cDNA sequence of GATA3 was amplified by reverse transcription polymerase chain reaction(RT-PCR).
方法:利用RTPCR方法,从Balb/c小鼠脾细胞的mRNA中扩增GATA3基因全长cDNA,并将其连接到pMD18-T载体上,进行测序和核酸分析。
2.
In present study, atherosclerotic animal model were replicated with high cholesterol feedstuff , and the expressions of estrogen receptor α and low-density lipoprotein receptor mRNA in heart and liver were studied by half quantitive RT-PCR, the rabbits oophorectomized and fed with normal feedstuff were used as control.
采用βactin做内参照的半定量RTPCR方法,对2组去势雌兔心脏、肝脏组织内雌激素受体α和低密度脂蛋白受体mRNA的表达进行了研究。
3.
Based on epidemical investigation, clinical symptom and pathology changes, both RT-PCR and PCR were used to detect porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type-2 (PCV-2) respectively.
在流行病学调查、临床症状、病理剖检的基础上,采用已建立的诊断猪繁殖与呼吸综合征病毒的RTPCR技术和诊断猪圆环病毒2型的PCR技术,从这几个规模化猪场的病料中均扩增出特异的目的基因片段,证实为猪繁殖与呼吸综合征病毒和猪圆环病毒2型混合感染。
3)  RT PCR
RTPCR
4)  dsRNA dependent ribonuclease
dsRNA依赖的核糖核酸酶
5)  Hypovirulence-associated dsRNA
与低毒力相结合的dsRNA
6)  dsRNA virus
dsRNA病毒
1.
ABSTRACT The genomes of dsRNA viruses infecting Pleurotus ostreatus were isolated from the mycelia of Pleurotus ostreatus and had 4 segments by the dsRNA technique.
本研究利用dsKNA技术,从平菇天达300菌丝体中提取出平菇dsRNA病毒的基因组,共有4个片段,大小分别为8。
2.
The dsRNA virus was detected in the Trichomonas vaginalis isolated from human in Changchun.
结果表明,在我国阴道毛滴虫长春分离株发现阴道毛滴虫病毒,该病毒属于dsRNA病毒。
补充资料:核糖核酸酶
分子式:
CAS号:

性质:催化RNA水解的一种核酸内切酶。反应产物是单核苷酸和寡核苷酸。不同核糖核酸酶催化产物的低聚核苷酸组成有差别。核糖核酸酶T1使之产生单核苷酸和以3′-鸟苷酸为组成的或末端为3′-鸟苷酸的低聚核苷酸。核糖核酸酶T2使之产生单核苷酸和以3′-腺苷酸为组成的或末端为3′-腺苷酸的低聚核苷酸。绝大部分的核糖核酸酶需要二价阳离子作为辅因子(例如Ca2+,Mg2+等)。因此活性可因乙二胺四乙酸(EDTA)的存在而阻断。胰核糖核酸酶是3′-嘧啶核苷酸由红酵母发酵液提制而得的核糖核酸酶,其药用油膏可局部外用于治疗外伤及关节疼痛。

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