1) overlapping PCR
重迭PCR
1.
Then, the fused translational expression vector PxylR_Cry1Ac was constructed using overlapping PCR technique with the primers pair PxyF/Cry1AcR and the mixture of above PCR production.
根据苏云金芽孢杆菌BacillusthuringiensisHD_73基因Cry1Ac和枯草芽孢杆菌Bacillussubtilis木糖诱导型启动子PxylR序列,分别设计2对特异引物Cry1AcFR和PxyFR,扩增获得了完整的启动子PxylR和Cry1Ac基因序列,进一步以上述产物混合物为模板,以PxyFCry1AcR作引物进行重迭PCR,获得了载体PxylR_Cry1Ac,经SphⅠ和BamHⅠ完全酶切后,将PxylR_Cry1Ac插入大肠杆菌_苏云金芽孢杆菌穿梭载体pHT315,重组表达质粒pCry1Ac315转化枯草芽孢杆菌感受态细胞。
2) overlap
[英][,əuvə'læp] [美][,ovɚ'læp]
重迭
1.
Birefringence measurements using overlapping of interference fringes;
晶体双折射测量的干涉条纹重迭法
3) multiplex PCR
多重PCR
1.
Detection of Salmonella spp.,Escherichia coli and Staphylococcus aureus by Multiplex PCR;
沙门菌、大肠杆菌和金黄色葡萄球菌的多重PCR检测
2.
Simultaneous Detection of Common Food-borne Bacterial Pathogens in Innocuous Poultry and Seafood by Multiplex PCR Assay;
多重PCR检测无公害畜禽肉和水产品中4种致病菌
3.
The clinical application of multiplex PCR in diagonsis of deafness with mtDNA mutation;
多重PCR诊断线粒体突变聋病的临床应用价值
4) Multiplex polymerase chain reaction
多重PCR
1.
Multiplex polymerase chain reaction assay for sex determination from a single cell.;
应用单细胞多重PCR进行性别鉴定
2.
Objective To develop a novel multiplex polymerase chain reaction (PCR) to detect multidrug-resistant Acinetobacter baumannii.
目的建立一种适于临床微生物实验室快速检测多耐药鲍曼不动杆菌基因的多重PCR方法。
3.
A multiplex polymerase chain reaction(PCR)-based method was designed for simultaneous detection of four main viruses involved in respiratory and/or reproductive failure in piglets(porcine circovirus type 2 ,porcine parvovirus ,porcine reproductive and respiratory syndrome virus ,and classical swine fever virus ).
本试验建立一种可同时检测猪圆环病毒2型(PCV-2),猪细小病毒(PPV),猪繁殖与呼吸综合征病毒(PRRSV),猪瘟病毒(CSFV)4种病毒的多重PCR方法。
5) multiple PCR
多重PCR
1.
Detection of hMSH2 gene mutations in gynecologic tumor population by multiple PCR-SSCP;
多重PCR-SSCP法检测妇科肿瘤患者hMSH2基因突变
2.
Detection of Herpes simplex Virus,Treponema pallidum and Haemophilus ducreyi by Multiple PCR
多重PCR同时检测苍白密螺旋体、单纯疱疹病毒及杜氏嗜血菌的实验研究
3.
Objective Using multiple PCR to screen the mutational status of the protease serine 1(PRSS1) gene and serine protease inhibitor Kazal type1(SPINK1) gene in chronic pancreatitis.
目的探讨多重PCR技术在筛查慢性胰腺炎患者的易感基因即胰蛋白酶原基因(protease serine 1,PRSS1)和胰腺囊性纤维化基因(serine protease inhibitor Kazal type1,SPINK1)突变的情况。
6) multiplex-PCR
多重PCR
1.
Study on Detection of Five-kinds of Pathogenic Bacteria in Drinking Water by Multiplex-PCR;
饮用水中5种致病菌多重PCR技术检测研究
2.
Multiplex-PCR Approach to Identify Bacillus megaterium and Bacillus cereus Group Applied in Microbial Fertilizers
多重PCR技术检测微生物肥料中巨大芽孢杆菌和蜡样群芽孢杆菌的研究与应用
3.
Establishment of Multiplex-PCR for Waxy Genes in Wheat
小麦糯性基因多重PCR体系的建立
补充资料：层层迭迭
1.见"层层迭迭"。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条