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1)  Duplex RT-PCR
双重RT-PCR
1.
One-step Duplex RT-PCR Assay for Detection SARS Associated Coronavirus;
一步法双重RT-PCR检测SARS冠状病毒技术研究
2.
Duplex RT-PCR Detection of Potato spindle tuber viroid with an Internal Control of Potato ND2 mRNA;
以马铃薯ND2 mRNA为内对照双重RT-PCR法检测马铃薯纺锤块茎类病毒
3.
Development of Duplex RT-PCR Protocol for Detection of Two Viruses in Orchid
兰花两种主要病毒双重RT-PCR检测方法的建立
2)  Duplex RT-PCR
双重RT-PCR方法
1.
Detection of Avian Influenza Virus and Newcastle Disease Virus by Duplex RT-PCR Technique;
检测新城疫病毒和禽流感病毒的双重RT-PCR方法的建立
3)  Double one-step RT-PCR
双重一步法RT-PCR
4)  Multiplex RT-PCR
多重RT-PCR
1.
Co-expression of MAGEA3,hSTC1,CK20,CK19 and CEA mRNA in Peripheral Blood of Patients with Esophageal Carcinoma by Multiplex RT-PCR;
应用多重RT-PCR技术检测食管癌患者外周血多种肿瘤基因标志物的研究
2.
Development of a multiplex RT-PCR to detect West Nile virus,Venezuelan equine encephalomyelitis virus and Hendra virus;
3种重要脑炎病毒的多重RT-PCR检测方法的建立
3.
Combination of red blood cell enrichment and multiplex RT-PCR to detect avian Influenza viruses;
红细胞富集与多重RT-PCR联合检测禽流感病毒
5)  duplex RT-PCR
二重RT-PCR
1.
Development and Application of A Duplex RT-PCR Method to Differentiate the PRRSV Variant and Common Strains
PRRSV变异株与普通株二重RT-PCR鉴别方法的建立与应用
2.
Developmemt of a duplex RT-PCR assay for detection of encephalomyocarditis virus and porcine reproductive and respiratory syndrome virus
猪脑心肌炎病毒和猪繁殖与呼吸综合征病毒二重RT-PCR检测方法的建立
6)  one-step multiplex RT-PCR
一步法多重RT-PCR
1.
Simultaneous detection of porcine circovirus type 2 and porcine respiratory and reproductive syndrome virus by one-step multiplex RT-PCR;
一步法多重RT-PCR检测猪圆环病毒2型和猪繁殖与呼吸道综合征病毒
补充资料:immune PCR
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性质: 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上,从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上,进而证明有抗原存在。目前最为敏感的检测方法,理论上可测得一个抗原分子的存在。

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