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1)  double PCR
两重PCR
1.
Development of methods on detection of enteropathogenic escherichia coli by double PCR in meat and meat product;
肉及肉制品中肠致病性大肠杆菌两重PCR检测方法的建立
2)  multipex nested PCR
两重巢式PCR
3)  Two-step RT-PCR
两步法RT-PCR
1.
Three different methods(Two-step RT-PCR,Access-quick RT- PCR system and QIAGEN One-step RT-PCR Kit)were used to ampli- fyβ-actin gene by reverse-transcription polymerase chain reaction(RT- PCR).
方法:采集13例新生大鼠脊髓标本,提取RNA,分别稀释至1μg/μl、1ng/μl和1 pg/μl,分别以两步法RT-PCR、Promega公司和Qiagen公司一步法RT-PCR试剂盒,扩增β-actin基因,经1。
4)  multiplex PCR
多重PCR
1.
Detection of Salmonella spp.,Escherichia coli and Staphylococcus aureus by Multiplex PCR;
沙门菌、大肠杆菌和金黄色葡萄球菌的多重PCR检测
2.
Simultaneous Detection of Common Food-borne Bacterial Pathogens in Innocuous Poultry and Seafood by Multiplex PCR Assay;
多重PCR检测无公害畜禽肉和水产品中4种致病菌
3.
The clinical application of multiplex PCR in diagonsis of deafness with mtDNA mutation;
多重PCR诊断线粒体突变聋病的临床应用价值
5)  Multiplex polymerase chain reaction
多重PCR
1.
Multiplex polymerase chain reaction assay for sex determination from a single cell.;
应用单细胞多重PCR进行性别鉴定
2.
Objective To develop a novel multiplex polymerase chain reaction (PCR) to detect multidrug-resistant Acinetobacter baumannii.
目的建立一种适于临床微生物实验室快速检测多耐药鲍曼不动杆菌基因的多重PCR方法。
3.
A multiplex polymerase chain reaction(PCR)-based method was designed for simultaneous detection of four main viruses involved in respiratory and/or reproductive failure in piglets(porcine circovirus type 2 ,porcine parvovirus ,porcine reproductive and respiratory syndrome virus ,and classical swine fever virus ).
本试验建立一种可同时检测猪圆环病毒2型(PCV-2),猪细小病毒(PPV),猪繁殖与呼吸综合征病毒(PRRSV),猪瘟病毒(CSFV)4种病毒的多重PCR方法。
6)  multiple PCR
多重PCR
1.
Detection of hMSH2 gene mutations in gynecologic tumor population by multiple PCR-SSCP;
多重PCR-SSCP法检测妇科肿瘤患者hMSH2基因突变
2.
Detection of Herpes simplex Virus,Treponema pallidum and Haemophilus ducreyi by Multiple PCR
多重PCR同时检测苍白密螺旋体、单纯疱疹病毒及杜氏嗜血菌的实验研究
3.
Objective Using multiple PCR to screen the mutational status of the protease serine 1(PRSS1) gene and serine protease inhibitor Kazal type1(SPINK1) gene in chronic pancreatitis.
目的探讨多重PCR技术在筛查慢性胰腺炎患者的易感基因即胰蛋白酶原基因(protease serine 1,PRSS1)和胰腺囊性纤维化基因(serine protease inhibitor Kazal type1,SPINK1)突变的情况。
补充资料:两重
1.谓两者一起被重用﹑倚重。
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