1) overlap PCR
重叠PCR
1.
Method In this study,the fusion gene ctxAB containing ctxA and ctxB gene was obtained from DNA of Vibrio cholera by overlap PCR,and to construct recombinant plasmid pET-ctxAB the fusion gene ctxAB was cloned into prokaryotic expressed vector pET32a(+) co.
方法以霍乱弧菌DNA为模板,利用重叠PCR技术,扩增出含有ctxA和ctxB的融合基因片段ctxAB,并与带有硫氧还蛋白(Trx)基因的高效原核表达质粒pET32a(+)定向重组,构建重组质粒,转化大肠杆菌BL21(DE3)。
2.
The human anti-HBsAg ScFv and interferon gamma were connected with the linker to construct the ScFv-IFNγ gene by using overlap PCR techniques.
采用重叠PCR技术 ,将人源性抗HBsAg单链抗体基因与干扰素 γ 基因用柔性肽段碱基连接成单一基因 。
3.
The H chain and L chain gene of anti_HBsAg fab were connected to construct single_chain fab gene using overlap PCR techniques.
采用重叠PCR技术 ,以抗乙肝表面抗原 (HBsAg)IgG铰链区基因为Linker将Fab抗体基因的重链和轻链连接起来 ,构成单链Fab基因。
2) Overlapping PCR
重叠PCR
1.
The gene fragments of two tandems were synthesized by overlapping PCR,inserted into pQE-30 vector,and transformed in Escherichia coli.
对其基因进行优化后,经重叠PCR方法合成串联体A、B的全长基因。
2.
Objective: For expression in Aspergillus awamori,the plant sweet protein brazzein gene was synthesized with overlapping PCR.
目的:为在泡盛曲霉(Aspergillus awamori)中进行表达,采用重叠PCR合成了植物甜蛋白brazzein基因。
3.
Then with overlapping PCR technology to link this two DNA together to form a complete ZP 3 gene encoding.
再经重叠PCR技术将这两段DNA连接在一起,构成完整的ZP 3基因的编码区。
3) Overlap extension PCR
重叠延伸PCR
1.
1 as the template,the PSD-95 mutant genes were amplified by overlap extension PCR,and then were cloned into the plasmids of PSD-95-pcDNA3.
1为模板,采用重叠延伸PCR法获得突变型PSD-95的局部或全长cDNA片段,并克隆至PSD-95-pcDNA3。
2.
According to the amino acids sequence of OC-IΔD86 gene and Escherichia coli codon usage, we synthesized this gene by overlap extension PCR method with 7 oligonucleotides DNA fragments.
根据OC-IΔD86基因序列,设计合成了7条寡核苷酸片段,通过重叠延伸PCR技术合成了OC-IΔD86基因,利用设计好的BamH I/Xho I酶切位点将OC-IΔD86基因克隆到原核表达载体pet21b中,在1mmol/L的IPTG诱导后5h,OC-I?D86融合基因在大肠杆菌中得到表达,表达产物处于可溶状态,其表达量占总蛋白的11。
3.
How to create two separate site-specific mutagenesis in a DNA fragment using overlap extension PCR was explored.
探讨如何利用重叠延伸PCR对同一靶DNA片段中的两个不同位点实施联合突变。
4) overlapping extension PCR
重叠延伸PCR
1.
Cell culture-adaptive mutations were carried out by the method of overlapping extension PCR OE-PCR at the sites of E1202G T1280I and S2197P in the genes of ns3 and ns5a.
方法:根据HCV全长基因组cDNA序列及突变位点设计引物,运用重叠延伸PCR法对ns3和ns5a基因的E1202G、T1280I和S2197P位点进行细胞培养适应性突变,将突变后的片段分别克隆入pBluescriptIIKS(+)、pRSET-A载体,经测序正确后,分别连入含有HCV全长cDNA的质粒的H/FL相应位置,置换原未突变片段,并进行PCR及酶切鉴定。
5) overlap extention PCR
重叠延伸PCR
1.
K88ac-STⅡ fused gene amplified by overlap extention PCR was cloned into T vector.
利用重叠延伸PCR技术将K88ac STⅡ融合基因克隆于T载体上,将重组基因质粒转化到受体菌DH10B中,蓝白斑筛选阳性菌落,通过PCR、NcoI/XcoI酶切后测序,与genbank报道的K88ac结构基因序列进行比对,证明所克隆的目的片段为K88ac STII融合基因。
6) splicing overlap extension PCR
重叠延伸PCR
1.
Methods: The VH-linker-VL, namely scFv was prepared by amplifying the VH and VL genes of plasmid pGEM-T-VH and pGEM-T-VL with splicing overlap extension PCR(SOE PCR).
方法:设计以SfiⅠ、NotⅠ为酶切位点、以(Gly4Ser)3为linker的2对引物,从抗人PAF单克隆抗体可变区基因的克隆载体中扩增VH和VL基因,用重叠延伸PCR在VH和VL基因间引入连接短肽,构建VH-linker-VL的scFv基因。
2.
Methods The VH-linker-VL, namely scFv gene was prepared by amplifying the VH and VL genes of plasmid pGEM-T-VH and pGEM-T-VL with splicing overlap extension PCR (SOE PCR).
方法 从抗TfR单克隆抗体重链和轻链可变区基因的克隆载体 pGEM T VH和 pGEM T VL中扩增重链可变区 (VH)和轻链可变区 (VL)基因 ,用重叠延伸PCR的方法 ,在VH和VL基因间引入连接短肽 (Linker) ,构建VH Linker VL的单链抗体 (singlechainFv ,scFv)基因。
3.
Then, the complete gene of AgB was cloned by splicing overlap extension PCR method using 35 nucleotides overlap between the upper and downer fragments and inserted into the vector pVAX1.
采用重叠延伸PCR法(splicing overlap extension PCR method,SOE-PCR)把具有35个相同碱基的上下半段扩增为全长基因,并构建到真核表达载体pVAX1,将酶切、PCR、测序鉴定的阳性质粒经脂质体转染BHK-21细胞。
补充资料：immune PCR
分子式：
CAS号：
性质： 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上，从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上，进而证明有抗原存在。目前最为敏感的检测方法，理论上可测得一个抗原分子的存在。
CAS号：
性质： 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上，从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上，进而证明有抗原存在。目前最为敏感的检测方法，理论上可测得一个抗原分子的存在。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条